Recombinant Histone H1 Antibody [clone r1415-1] (V3735)

Description

Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.

Application Notes

Optimal dilution of the recombinant Histone H1 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
2. View the non-recombinant version of this Histone H1 antibody.

Immunogen

Nuclei of human leukemia biopsy cells were used as the immunogen for the recombinant Histone H1 antibody.

Storage

Store the recombinant Histone H1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).