- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Histone H1 Antibody / Replication-Coupled Chromatin Assembly Antibody [clone AE-4] (V7837)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V7837-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 559 | |
|
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V7837-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 259 | |
|
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V7837SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human, Mouse, Rat |
| Format | Purified |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Clone Name | AE-4 |
| Purity | Protein A affinity chromatography |
| UniProt | P07305 |
| Localization | Nuclear |
| Applications | Flow Cytometry : 1-2ug/million cells in 0.1ml Immunofluorescence : 1-2ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml Western Blot : 1-2ug/ml |
| Limitations | This recombinant Histone H1 antibody is available for research use only. |
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Histone H1 is a linker histone that plays a critical role in chromatin organization during DNA replication and replication-coupled chromatin assembly. Histone H1 Antibody detects H1 protein associated with newly assembled chromatin, where linker histones stabilize nucleosome arrays and contribute to the re-establishment of higher-order chromatin structure following DNA synthesis. This positioning highlights histone H1 as an important structural component in maintaining chromatin integrity during genome duplication. This antibody is part of our broader Histone H1 antibody collection, including linker histone variants, chromatin organization, chromatin accessibility, and nuclear architecture reagents for chromatin and epigenetics research.
Histone H1 antibody, also referred to as H1 antibody or linker histone antibody in the literature, is widely used to study chromatin organization during S-phase. During DNA replication, newly synthesized DNA must be rapidly assembled into chromatin through coordinated deposition of core histones followed by incorporation of linker histones. Histone H1 contributes to this process by binding to linker DNA and organizing nucleosome arrays into structured chromatin.
Clone AE-4 antibody is available in both a traditional mouse monoclonal format and a recombinant rabbit monoclonal format. The recombinant rabbit version of clone AE-4 is designed to provide improved consistency and reproducibility while maintaining the binding characteristics of the original mouse clone. This dual format can introduce naming overlap, but both versions are directed against histone H1 and support studies of chromatin organization.
Mechanistically, histone H1 is incorporated into chromatin following nucleosome assembly, where it stabilizes linker DNA and promotes interactions between adjacent nucleosomes. This facilitates the transition from newly assembled nucleosome arrays to more structured chromatin configurations. Proper incorporation of histone H1 is essential for maintaining chromatin organization during replication.
Replication-coupled chromatin assembly requires precise coordination between histone deposition and chromatin organization. Histone H1 plays a key role in this process by ensuring that newly assembled chromatin adopts a stable and functional structure. Disruption of H1 incorporation can lead to altered chromatin organization and compromise genome stability.
In contrast to roles focused on static chromatin structure, the function of histone H1 in replication emphasizes its involvement in dynamic chromatin reorganization. This makes it particularly relevant for studies of proliferating cells and chromatin assembly mechanisms during the cell cycle.
A recombinant rabbit monoclonal antibody such as clone AE-4 enables consistent detection of histone H1 in replication-associated chromatin, supporting studies of chromatin assembly, genome duplication, and cell cycle-dependent chromatin organization.
Optimal dilution of the Histone H1 Antibody / Replication-Coupled Chromatin Assembly Antibody should be determined by the researcher.
Nuclei of human leukemia biopsy cells were used as the immunogen for this Histone H1 Antibody / Replication-Coupled Chromatin Assembly Antibody.
Store the recombinant Histone H1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Histone H1 antibody, H1 antibody, replication chromatin histone H1 antibody, S-phase chromatin H1 antibody
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