His Tag Antibody / Polyhistidine Tag Antibody [clone RM146] (R20255)

Description

His Tag Antibody is an essential reagent for detecting recombinant proteins engineered with a polyhistidine affinity tag. The His tag, most commonly consisting of six consecutive histidine residues, is one of the most widely used fusion tags in molecular biology because it enables straightforward purification, detection, and characterization of recombinant proteins expressed in bacterial, yeast, insect, or mammalian systems. A high-quality His Tag Antibody allows researchers to verify protein expression, monitor purification workflows, confirm molecular weight by Western blotting, and visualize tagged proteins in numerous immunoassays. Clone RM146 is a recombinant rabbit monoclonal antibody developed to provide high affinity, excellent specificity, and reliable lot-to-lot consistency for these demanding research applications. NSJ Bioreagents offers this recombinant antibody for research use across a broad range of recombinant protein studies.

Polyhistidine tags are typically introduced at either the amino terminus or carboxyl terminus of a recombinant protein without substantially affecting protein folding or biological activity. The positively charged histidine residues coordinate efficiently with nickel or cobalt ions, making immobilized metal affinity chromatography a standard purification method for His-tagged proteins. Following purification, researchers commonly use a His Tag Antibody to verify successful expression, assess sample purity, detect degradation products, and compare expression levels between constructs. Because the same epitope can be incorporated into thousands of unrelated recombinant proteins, a single Polyhistidine Tag Antibody provides exceptional versatility and eliminates the need to generate target-specific antibodies during early stages of protein development.

Beyond routine Western blotting, a His Tag Antibody is frequently used in immunoprecipitation, immunofluorescence, immunohistochemistry, ELISA, flow cytometry, and other antibody-based detection methods when compatible protocols have been established. Detection of the common His tag simplifies validation of recombinant protein localization, protein-protein interactions, purification efficiency, and transgene expression in both basic and applied research. Recombinant rabbit monoclonal antibodies such as clone RM146 combine the high affinity typically associated with rabbit antibodies with the reproducibility expected from monoclonal reagents, making them valuable for laboratories seeking dependable performance across multiple experiments and production lots.

The widespread adoption of polyhistidine tagging throughout biotechnology, structural biology, enzymology, cell biology, and protein engineering has made the His Tag Antibody one of the most frequently used laboratory reagents for recombinant protein research. Whether confirming successful cloning, optimizing protein production, validating purification, or detecting tagged proteins in downstream functional assays, a reliable Polyhistidine Tag Antibody provides consistent, sensitive detection while supporting reproducible experimental results. Clone RM146 offers researchers a robust recombinant rabbit monoclonal solution for identifying His-tagged proteins across a diverse range of expression systems.

Explore our Cellular Marker & Epitope Tag Antibodies page to discover additional antibodies for detecting recombinant protein tags, cellular markers, and commonly used research epitopes.

Application Notes

The stated application concentrations are suggested starting points. Titration of the His Tag Antibody / Polyhistidine Tag Antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Immunogen

Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus was used as the immunogen for this recombinant His Tag antibody.

Storage

Store the recombinant His Tag antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Alternate Names

His Tag antibody, Polyhistidine Tag antibody, Histidine Tag antibody, 6xHis Tag antibody, Hexahistidine Tag antibody, His6 Tag antibody, His Epitope Tag antibody, Poly-His Tag antibody