- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Recombinant His Tag Antibody / Histidine [clone r6HIS/6423] (V9152)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V9152-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 559 | |
|
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V9152-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 259 | |
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V9152SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Species independent |
| Format | Purified |
| Host | Mouse |
| Clonality | Recombinant Mouse Monoclonal |
| Isotype | Mouse IgG1, kappa |
| Clone Name | r6HIS/6423 |
| Purity | Protein A/G affinity |
| Applications | Western Blot : 1-2ug/ml Flow Cytometry : 1-2ug/million cells Immunofluorescence : 1-2ug/ml |
| Limitations | This recombinant His Tag antibody is available for research use only. |
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Recombinant His Tag antibody recognizes the polyhistidine sequence, commonly referred to as His-tag or 6xHis tag, a short amino acid motif widely engineered into recombinant proteins for purification and detection. His Tag antibody, also referred to as anti-His antibody and 6xHis antibody in the literature, detects histidine-tagged fusion proteins expressed in bacterial, mammalian, or insect expression systems. Clone r6HIS/6423 is produced as a recombinant mouse monoclonal antibody, providing defined specificity and consistent performance for detection of His-tagged proteins in research applications.
The polyhistidine tag typically consists of six consecutive histidine residues positioned at the N- or C-terminus of a recombinant protein. This tag enables purification using immobilized metal affinity chromatography and facilitates immunodetection in downstream assays. Because the His-tag is relatively small and generally does not interfere with protein folding or function, it remains one of the most commonly used affinity tags in molecular biology and protein engineering workflows.
Detection of His-tagged proteins is essential for confirming expression, verifying purification, and evaluating protein integrity. In denatured lysates, intact fusion proteins are usually detected at their predicted molecular weight, while truncated or degraded species may also be observed depending on expression system and stability. Reliable recognition of the polyhistidine epitope is therefore critical for accurate interpretation of recombinant protein experiments.
Clone r6HIS/6423 recognizes the polyhistidine sequence independently of the fused protein backbone, supporting broad detection across diverse recombinant constructs. The recombinant mouse monoclonal format supports lot-to-lot consistency and defined epitope recognition, enabling reproducible results across independent experiments.
Recombinant His Tag antibody (clone r6HIS/6423) is suitable for research applications focused on detection, characterization, and analysis of His-tagged recombinant proteins in expression and purification systems.
Optimal dilution of the recombinant His Tag antibody should be determined by the researcher.
Hexa-histidine tagged human CD70 recombinant protein was used as the immunogen for the recombinant His Tag antibody.
Aliquot the recombinant His Tag antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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