His Tag Antibody Biotin Conjugate [clone RM146] (R20255BTN)

Description

His Tag Antibody Biotin Conjugate is designed for the sensitive detection of recombinant proteins carrying a polyhistidine affinity tag while providing the flexibility of biotin-streptavidin detection systems. The widely used His tag, typically composed of six consecutive histidine residues, enables efficient purification and universal detection of recombinant proteins produced in bacterial, yeast, insect, and mammalian expression systems. Biotin labeling allows this recombinant rabbit monoclonal antibody, clone RM146, to be paired with streptavidin-based detection reagents for enhanced assay versatility and signal amplification. NSJ Bioreagents provides this reagent for research applications requiring highly specific recognition of His-tagged recombinant proteins.

Biotin-conjugated antibodies are particularly valuable when investigators require flexible detection formats or wish to avoid species-specific secondary antibodies. Following binding of the His Tag Antibody Biotin Conjugate, streptavidin conjugated to horseradish peroxidase, alkaline phosphatase, fluorophores, or other reporter molecules can be selected according to the experimental design. This approach is commonly used in ELISA, immunohistochemistry, immunocytochemistry, Western blotting, flow cytometry, and multiplex immunoassays. Because the same polyhistidine epitope is shared by numerous recombinant proteins, a single biotinylated antibody can support diverse expression, purification, and characterization workflows.

Clone RM146 combines the advantages of recombinant rabbit monoclonal technology with the convenience of direct biotin conjugation. Recombinant production provides excellent lot-to-lot consistency while preserving the high affinity and specificity expected from rabbit monoclonal antibodies. The biotin label also simplifies assay development by reducing the number of incubation steps in many protocols and allowing straightforward integration into streptavidin-based detection systems. These features make the reagent well suited for applications involving recombinant protein validation, affinity purification studies, interaction analyses, and expression screening.

Whether confirming recombinant protein expression, developing sensitive immunoassays, or establishing streptavidin-based detection methods, His Tag Antibody Biotin Conjugate offers dependable recognition of polyhistidine-tagged proteins across multiple experimental platforms. Researchers seeking an unconjugated version for conventional secondary antibody detection can also explore our His Tag Antibody / Polyhistidine Tag Antibody page, featuring the same recombinant rabbit monoclonal clone RM146.

Application Notes

The stated application concentrations are suggested starting points. Titration of the His Tag Antibody Biotin Conjugate may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Immunogen

Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus was used as the immunogen for this biotinylated His Tag antibody.

Storage

Store the recombinant His Tag antibody at -20oC.

Alternate Names

Biotin His Tag antibody, Biotin Polyhistidine Tag antibody, Biotin Histidine Tag antibody, Biotin 6xHis Tag antibody, Biotin Hexahistidine Tag antibody, Biotin His6 Tag antibody, Biotin His Epitope Tag antibody, Biotin Poly-His Tag antibody