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Home >> Antibodies >> Recombinant HIF1A Antibody

Recombinant HIF1A Antibody [clone RM242] (R20263)

  Catalog No Formulation Size Price (USD)  
Image R20263-0.1ML Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ul 419
Microvalidated Recrabbitmono
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Western blot test of Jurkat cell lysate, untreated or treated with Cobalt Chloride (CoCl2), using recombinant HIF1A antibody at 1:1000. Routinely observed molecular weight: 100~120 kDa.
IHC testing of FFPE human breast cancer tissue with recombinant HIF1A antibody at 1:1000.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM242
Purity Protein A purified from animal origin-free supernatant
UniProt Q16665
Gene ID 3091
Localization Nuclear, possible cytoplasmic
Applications Immunohistochemistry (FFPE) : 1:500-1:1000 (1)
Western blot : 1:1000-1:2000
Limitations This recombinant HIF1A antibody is available for research use only.
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This recombinant HIF1A antibody reacts to human Hypoxia-inducible factor 1-alpha.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant HIF1A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.


A peptide corresponding to Hypoxia-inducible factor 1-alpha was used as the immunogen for this recombinant HIF1A antibody.


Store the recombinant HIF1A antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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