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Home >> Antibodies >> Recombinant HER2 Antibody

Recombinant HER2 Antibody [clone RM228] (R20258)

  Catalog No Formulation Size Price (USD)  
Image R20258-0.1ML Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 0.1 ml 419
Microvalidated Recrabbitmono
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Western blot of MCF7, PC3, Jurkat, and A375 cell lysate using recombinant HER2 antibody. Only the breast cancer line MCF7 showed a response.
IHC testing of FFPE human breast cancer tissue with recombinant HER2 antibody. Expected molecular weight: ~138 kDa (unmodified), ~185 kDa (glycosylated).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM228
Purity Protein A purified from animal origin-free supernatant
UniProt P04626
Gene ID 2064
Localization Cell surface, cytoplasm, nucleus
Applications Immunohistochemistry (FFPE) : 1:100-1:400 (1)
Western blot : 1:1000
Limitations This recombinant HER2 antibody is available for research use only.
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Description

This recombinant HER2 antibody reacts to Human c-erbB-2/HER-2. It may also react to mouse or rat c-erbB-2/HER-2, as predicted by immunogen homology.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant HER2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Immunogen

A peptide corresponding to the C-terminus of human c-erbB2/HER2 was used as the immunogen for this recombinant HER2 antibody.

Storage

Store the recombinant HER2 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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