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Home >> Antibodies >> Recombinant HER2 Antibody / ErbB2

Recombinant HER2 Antibody / ErbB2 [clone ERBB2/8142R] (V4469)

  Catalog No Formulation Size Price (USD)  
Image V4469-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4469-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4469SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human breast carcinoma tissue with recombinant HER2 antibody (clone ERBB2/8142R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast carcinoma tissue with recombinant HER2 antibody (clone ERBB2/8142R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name ERBB2/8142R
Purity Protein A/G affinity
UniProt P04626
Localization Cell surface
Applications Flow cytometry : 1-2ug/million cells
Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This recombinant HER2 antibody is available for research use only.
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Description

C-erbB-2/HER-2 is a member of the EGFR family. This mAb is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.

Application Notes

Optimal dilution of the recombinant HER2 antibody should be determined by the researcher.

Immunogen

A recombinant partial protein sequence (within amino acids 1055-1255) from the human protein was used as the immunogen for the recombinant HER2 antibody.

Storage

Aliquot the recombinant HER2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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