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Home >> Antibodies >> Recombinant HA Tag Chimeric Human Antibody

Recombinant HA Tag Chimeric Human Antibody [clone RMH02] (R20305)

  Catalog No Formulation Size Price (USD)  
Image R20305-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 379
Western blot of 293T cells transfected (+) or non-transfected (-) with a DNA construct encoding HA-Tag KRas fusion protein, using recombinant HA Tag Chimeric Human antibody at 0.1 ug/ml, followed by a HRP conjugated anti-Human IgG secondary.
Sandwich ELISA performed on whole lysate of human LNCaP cells overexpressing HA-tag Bag1 protein. ELISA plate was coated with 50uL/well of recombinant HA Tag Chimeric Human antibody (1ug/ml) as the capture. Anti-Bag1 rabbit serum was used as the detect, followed by an alkaline phosphatase conjugated anti-Rabbit IgG as the secondary Ab.
Availability 1-3 business days
Species Reactivity NA
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RMH02
Purity Protein A purified from animal origin-free supernatant
UniProt N/A
Applications ELISA (Capture) : 0. 5 ug/ml-2 ug/ml
ELISA (Detect) : 0.01 ug/ml-0.5 ug/ml
Western Blot : 0.1 ug/ml-1 ug/ml
Limitations This recombinant HA Tag Chimeric Human antibody is available for research use only.
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Description

This antibody reacts to recombinant proteins containing a HA-Tag fused to either the amino or carboxy terminus. An anti-human IgG secondary is required for this mAb. No cross reactivity with any endogenous protein.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant HA Tag Chimeric Human antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A peptide corresponding to HA-tag (Human influenza hemagglutinin amino acids 98-106) was used as the immunogen for the recombinant HA Tag Chimeric Human antibody.

Storage

Store the recombinant HA Tag Chimeric Human antibody at -20oC.

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