- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
H4K8ac Antibody / Histone H4 Lysine 8 Acetylation Chromatin Remodeling Antibody [clone RM201] (R20225)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
R20225-100UG | 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide | 100 ug | 485 | |
|
|
R20225-25UG | 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide | 25 ug | 259 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Clone Name | RM201 |
| Purity | Protein A purified from animal origin-free supernatant |
| UniProt | P62805 |
| Gene ID | 121504 |
| Applications | Western Blot : 0.5-2ug/ml ELISA : 0.2-1ug/ml Immunocytochemistry : 0.5-2ug/ml |
| Limitations | This recombinant H4K8ac antibody is available for research use only. |
|
Review this product on BioCompare and get a $20 Amazon gift card
|
Related Products
|
Histone H4 (HIST1H4) is a core nucleosomal histone whose lysine 8 acetylation (H4K8ac) contributes to dynamic chromatin remodeling and increased DNA accessibility. H4K8ac Antibody (clone RM201) detects this modification, which is associated with nucleosome mobility and structural transitions that allow chromatin to shift between compact and accessible states. This positions H4K8ac as a key marker of chromatin remodeling rather than static chromatin organization. This antibody is part of our broader Histone H4 antibody collection, including acetylation, methylation, phosphorylation, and total H4 detection reagents for chromatin and epigenetics research.
H4K8ac antibody, also referred to as Histone H4 K8 acetyl antibody or HIST1H4 acetylation antibody in the literature, is commonly used to study chromatin accessibility and remodeling processes. Acetylation at lysine 8 is catalyzed by histone acetyltransferases such as p300 and CBP, which are recruited to regions of chromatin undergoing structural reorganization. This modification promotes recruitment of bromodomain-containing proteins that recognize acetylated lysine residues and facilitate chromatin remodeling.
Mechanistically, acetylation at lysine 8 neutralizes the positive charge of the histone tail, weakening histone-DNA interactions and promoting nucleosome repositioning. This enhances chromatin flexibility and allows regulatory complexes to access DNA. Unlike lysine 5 acetylation, which is linked to newly synthesized histones, H4K8 acetylation is more strongly associated with dynamic chromatin remodeling events occurring throughout the genome.
The histone H4 tail plays a central role in mediating interactions between nucleosomes, and acetylation at lysine 8 disrupts these interactions to facilitate chromatin reorganization. This results in increased chromatin accessibility without necessarily inducing large-scale chromatin unfolding, distinguishing H4K8ac from modifications that regulate higher-order chromatin structure.
H4K8 acetylation also contributes to recruitment of chromatin remodeling complexes and histone readers that drive nucleosome repositioning and chromatin restructuring. Dysregulation of this modification has been linked to altered epigenetic states and aberrant chromatin accessibility in disease contexts, including cancer.
A recombinant rabbit monoclonal antibody such as clone RM201 enables specific detection of H4K8 acetylation in studies focused on chromatin remodeling, nucleosome mobility, and accessibility-driven chromatin dynamics.
The stated application concentrations are suggested starting points. Titration of the H4K8ac Antibody / Histone H4 Lysine 8 Acetylation Chromatin Remodeling Antibody may be required due to differences in protocols and secondary/substrate sensitivity.
An acetyl-peptide corresponding to Acetyl-Histone H4 (Lys8) was used as the immunogen for this H4K8ac Antibody / Histone H4 Lysine 8 Acetylation Chromatin Remodeling Antibody.
Store the recombinant H4K8ac antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
H4K8ac antibody, Histone H4 acetyl lysine 8 antibody, HIST1H4 acetylation antibody, H4 chromatin remodeling antibody
Your bulk quote request has been submitted successfully!
Please contact us if you have any questions.
Powered by Bioz
