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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
H4K5ac Antibody / Histone H4 Lysine 5 Acetylation Chromatin Assembly Antibody [clone RM199] (R20224)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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R20224-100UG | 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide | 100 ug | 485 | |
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R20224-25UG | 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide | 25 ug | 259 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Clone Name | RM199 |
| Purity | Protein A purified from animal origin-free supernatant |
| UniProt | P62805 |
| Gene ID | 121504 |
| Applications | Western Blot : 0.2-1ug/ml ELISA : 0.2-1ug/ml Immunocytochemistry : 0.5-2ug/ml |
| Limitations | This recombinant H4K5ac antibody is available for research use only. |
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Histone H4 (HIST1H4) is a core nucleosomal histone whose lysine 5 acetylation (H4K5ac) marks newly synthesized histones during replication-coupled chromatin assembly. H4K5ac Antibody (clone RM199) detects this modification, which is tightly linked to nucleosome deposition and early chromatin formation. This acetylation event is one of the defining features of newly incorporated histone H4 and provides a direct readout of chromatin assembly activity rather than established chromatin states. This antibody is part of our broader Histone H4 antibody collection, including acetylation, methylation, phosphorylation, and total H4 detection reagents for chromatin and epigenetics research.
H4K5ac antibody, also referred to as Histone H4 K5 acetyl antibody or HIST1H4 acetylation antibody in the literature, is widely used to investigate replication-associated chromatin dynamics and histone deposition pathways. Acetylation at lysine 5 is catalyzed primarily by the histone acetyltransferase HAT1, which modifies newly synthesized histone H4 prior to its incorporation into chromatin. This modification, often occurring together with K12 acetylation, facilitates proper histone chaperone interactions and nucleosome assembly.
Functionally, acetylation at lysine 5 neutralizes the positive charge of the histone tail, reducing its affinity for DNA and allowing flexible nucleosome formation. This promotes efficient chromatin assembly by enabling histones to be deposited onto DNA without premature compaction. In contrast to methylation-based modifications that stabilize chromatin structure, H4K5 acetylation supports a transiently open chromatin state required for nucleosome positioning and chromatin maturation.
During DNA replication, newly synthesized histones bearing H4K5ac are incorporated into chromatin and subsequently undergo deacetylation as chromatin matures. This transient nature makes H4K5ac a highly informative marker of chromatin assembly timing, histone turnover, and replication-coupled nucleosome formation. Elevated or prolonged acetylation at this site can reflect disruptions in chromatin maturation and has been associated with genome instability.
From a structural perspective, the histone H4 tail contributes to nucleosome organization and inter-nucleosomal interactions, and acetylation at lysine 5 temporarily modulates these interactions to allow proper chromatin formation. This distinguishes H4K5ac from other H4 modifications that are involved in long-term chromatin compaction or repression.
A recombinant rabbit monoclonal antibody such as clone RM199 enables specific detection of H4K5 acetylation in studies focused on chromatin assembly, replication dynamics, histone deposition, and early-stage chromatin organization.
The stated application concentrations are suggested starting points. Titration of the H4K5ac Antibody / Histone H4 Lysine 5 Acetylation Chromatin Assembly Antibody may be required due to differences in protocols and secondary/substrate sensitivity.
An acetyl-peptide corresponding to Acetyl-Histone H4 (Lys5) was used as the immunogen for this H4K5ac Antibody / Histone H4 Lysine 5 Acetylation Chromatin Assembly Antibody.
Store the recombinant H4K5ac antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
H4K5ac antibody, Histone H4 K5 acetyl antibody, HIST1H4 acetylation antibody, H4 chromatin assembly antibody
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