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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
H4K20ac Antibody / Histone H4 Lysine 20 Acetylation Chromatin Structure Antibody [clone RM205] (R20227)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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R20227-100UG | 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide | 100 ug | 485 | |
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R20227-25UG | 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide | 25 ug | 259 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Clone Name | RM205 |
| Purity | Protein A purified from animal origin-free supernatant |
| UniProt | P62805 |
| Gene ID | 121504 |
| Applications | Western Blot : 1-2ug/ml ELISA : 0.2-1ug/ml Immunocytochemistry : 1-2ug/ml |
| Limitations | This recombinant H4K20ac antibody is available for research use only. |
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Histone H4 (HIST1H4) is a core nucleosomal histone whose lysine 20 acetylation (H4K20ac) represents a distinct regulatory modification influencing chromatin structure and accessibility. H4K20ac Antibody (clone RM205) detects acetylation at lysine 20, a residue more commonly associated with methylation-driven chromatin compaction. Acetylation at this site introduces an alternative regulatory state that contrasts with methylation-dependent chromatin condensation and reflects a shift toward a more accessible chromatin configuration. This antibody is part of our broader Histone H4 antibody collection, including acetylation, methylation, phosphorylation, and total H4 detection reagents for chromatin and epigenetics research.
H4K20ac antibody, also referred to as Histone H4 K20 acetyl antibody or HIST1H4 acetylation antibody in the literature, is used to investigate chromatin states where acetylation counterbalances methylation-mediated chromatin compaction. This modification alters the functional output of lysine 20 by neutralizing its positive charge, thereby reducing nucleosome interaction strength and promoting localized chromatin accessibility.
Mechanistically, acetylation at lysine 20 disrupts the same structural interactions that are enhanced by H4K20 methylation. This creates a regulatory switch at a single residue, allowing chromatin to transition between compact and accessible states depending on the modification present. As such, H4K20ac is particularly valuable for studying chromatin state transitions and the balance between chromatin condensation and relaxation.
The histone H4 tail is critical for mediating interactions between nucleosomes, and modification at lysine 20 has a direct impact on these interactions. Acetylation reduces chromatin fiber compaction and contributes to a more open chromatin configuration, distinguishing H4K20ac from methylation states that reinforce chromatin density. This makes H4K20ac a useful marker for examining chromatin boundary regions and transitional chromatin states.
H4K20 acetylation may also influence recruitment of chromatin-associated proteins that recognize acetylated lysine residues, further contributing to changes in chromatin organization. Dysregulation of this balance between acetylation and methylation at lysine 20 has been implicated in altered epigenetic states and disease processes, including cancer.
A recombinant rabbit monoclonal antibody such as clone RM205 enables specific detection of H4K20 acetylation in studies focused on chromatin structure, chromatin state transitions, and epigenetic regulation of genome organization.
The stated application concentrations are suggested starting points. Titration of the H4K20ac Antibody / Histone H4 Lysine 20 Acetylation Chromatin Structure Antibody may be required due to differences in protocols and secondary/substrate sensitivity.
An acetyl-peptide corresponding to Acetyl-Histone H4 (Lys20) was used as the immunogen for this H4K20ac Antibody / Histone H4 Lysine 20 Acetylation Chromatin Structure Antibody.
Store the recombinant H4K20ac antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
H4K20ac antibody, Histone H4 acetyl lysine 20 antibody, HIST1H4 acetylation antibody, H4 chromatin boundary antibody
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