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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
H4K12ac Antibody / Histone H4 Lysine 12 Acetylation Chromatin Assembly Antibody [clone RM202] (R20230)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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R20230-100UG | 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide | 100 ug | 485 | |
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R20230-25UG | 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide | 25 ug | 259 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Clone Name | RM202 |
| Purity | Protein A purified from animal origin-free supernatant |
| UniProt | P62805 |
| Gene ID | 121504 |
| Applications | Western Blot : 0.5-2ug/ml ELISA : 0.5-1ug/ml Immunocytochemistry : 0.5-2ug/ml |
| Limitations | This recombinant H4K12ac antibody is available for research use only. |
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Histone H4 (HIST1H4) is a core nucleosomal histone whose lysine 12 acetylation (H4K12ac) is a defining feature of newly synthesized histones during replication-coupled chromatin assembly. H4K12ac Antibody (clone RM202) detects this modification, which functions together with lysine 5 acetylation to mark histone H4 prior to its incorporation into chromatin. This coordinated acetylation pattern provides a highly specific signature of histone deposition and early nucleosome formation. This antibody is part of our broader Histone H4 antibody collection, including acetylation, methylation, phosphorylation, and total H4 detection reagents for chromatin and epigenetics research.
H4K12ac antibody, also referred to as Histone H4 K12 acetyl antibody or HIST1H4 acetylation antibody in the literature, is widely used to study chromatin assembly pathways and histone deposition dynamics. Acetylation at lysine 12 is catalyzed by histone acetyltransferase HAT1 and occurs on newly synthesized histone H4 in the cytoplasm before nuclear import. This modification facilitates interaction with histone chaperones and supports efficient nucleosome assembly during DNA replication.
Functionally, acetylation at lysine 12 neutralizes the positive charge of the histone tail, reducing its affinity for DNA and enabling flexible chromatin assembly. This creates a permissive structural environment that allows histones to be deposited onto DNA without premature chromatin compaction. In contrast to modifications that stabilize chromatin structure, H4K12 acetylation supports dynamic chromatin formation and is removed as chromatin matures.
The histone H4 tail plays a central role in nucleosome organization, and modification at lysine 12 contributes to proper nucleosome spacing and chromatin fiber establishment. H4K12ac is therefore closely linked to early chromatin maturation stages and provides insight into replication-associated chromatin dynamics rather than long-term chromatin structure or accessibility.
Following deposition, histones marked by H4K12ac undergo deacetylation as chromatin transitions into a more stable and organized state. Disruption of this process can lead to defects in nucleosome assembly, replication stress, and genome instability. As a result, H4K12ac serves as a valuable marker for studying histone turnover, replication timing, and chromatin assembly fidelity.
A recombinant rabbit monoclonal antibody such as clone RM202 enables specific detection of H4K12 acetylation in studies focused on chromatin assembly, nucleosome deposition, and replication-coupled chromatin organization.
The stated application concentrations are suggested starting points. Titration of the H4K12ac Antibody / Histone H4 Lysine 12 Acetylation Chromatin Assembly Antibody may be required due to differences in protocols and secondary/substrate sensitivity.
An acetyl-peptide corresponding to Acetyl-Histone H4 (Lys12) was used as the immunogen for this H4K12ac Antibody / Histone H4 Lysine 12 Acetylation Chromatin Assembly Antibody.
Store the recombinant H4K12ac antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
H4K12ac antibody, Histone H4 acetyl lysine 12 antibody, HIST1H4 acetylation antibody, H4 chromatin assembly acetylation antibody
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