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Home >> Antibodies >> Recombinant H3K9me3/S10p Antibody

Recombinant H3K9me3/S10p Antibody [clone RM162] (R20236)

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  Catalog No Formulation Size Price (USD)  
Image R20236-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 469
Image
R20236-25UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 25 ug 239
Microvalidated Recrabbitmono
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Western blot test of acid extracts of HeLa cells (1) and recombinant Histone H3.3 (2), using recombinant H3K9me3/S10p antibody at 0.01 ug/ml, showed a band of Histone H3 modified by both trimethylation at Lysine 9 and phosphorylation at Serine 10 (K9me3/S10p) in HeLa cells.
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Western blot test of acid extracts of HeLa cells (1) and recombinant Histone H3.3 (2), using recombinant H3K9me3/S10p antibody at 0.01 ug/ml, showed a band of Histone H3 modified by both trimethylation at Lysine 9 and phosphorylation at Serine 10 (K9me3/S10p) in HeLa cells.
This recombinant H3K9me3/S10p antibody specifically reacts to Histone H3 only when modified by both trimethylation at Lysine 9 and phosphorylation at Serine 10 (K9me3/S10p). No cross reactivity with non-modified Lysine 9/ Serine 10, methylated Lysine 9 ONLY, or phospho-Serine 9 ONLY in Histone H3.
Availability 1-3 business days
Species Reactivity All Species
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM162
Purity Protein A purified from animal origin-free supernatant
UniProt P84243
Gene ID 8350
Applications Western blot : 0.01ug/ml-1ug/ml
ELISA : 0.01ug/ml-0.5ug/ml
Limitations This recombinant H3K9me3/S10p antibody is available for research use only.
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Description

This recombinant H3K9me3/S10p antibody reacts to Histone H3 only when modified at both trimethyl Lysine 9 and phospho Serine 10 (K9me3/S10p).

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant H3K9me3/S10p antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A trimethyl-phospho-peptide corresponding to Trimethyl- Phospho-Histone H3 (Lys9/Ser10) was used as the immunogen for this recombinant H3K9me3/S10p antibody.

Storage

Store the recombinant H3K9me3/S10p antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Related Products

   1.   View all Recombinant antibodies

   2.   View all H3K9me3/S10p antibodies

   3.   View all Histone H3 antibodies

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