- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
H3K9me2 Antibody / HIST1H3A Stable Repressive Chromatin Antibody [clone RM151] (R20211)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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R20211-100UG | 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide | 100 ug | 485 | |
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R20211-25UG | 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide | 25 ug | 259 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Clone Name | RM151 |
| Purity | Protein A purified from animal origin-free supernatant |
| UniProt | P84243 |
| Gene ID | 8350 |
| Applications | Western Blot : 0.25-1ug/ml ChIP : 2-10ug/mg of lysate Immunocytochemistry : 0.5-2ug/ml ELISA : 0.2-1ug/ml |
| Limitations | This H3K9me2 antibody is available for research use only. |
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Histone H3 (HIST1H3A) methylation at lysine 9 is a central epigenetic modification governing chromatin repression and heterochromatin formation. Dimethylation at lysine 9 represents a stable repressive chromatin state associated with facultative heterochromatin and sustained gene silencing across large genomic domains. H3K9me2 Antibody / HIST1H3A Stable Repressive Chromatin Antibody (clone RM151) is designed to detect Histone H3 dimethylated at lysine 9, providing a robust marker of chromatin regions undergoing persistent transcriptional repression. This antibody is part of a broader collection of Histone H3 antibodies used to study chromatin structure, histone modifications, and epigenetic regulation.
HIST1H3A antibody, also referred to as Histone H3 antibody and H3K9me2 antibody in the literature, recognizes a modification that is more stable than H3K9me1 yet more dynamically regulated than H3K9me3. While H3K9me3 defines constitutive heterochromatin such as pericentromeric regions, H3K9me2 is broadly distributed across facultative heterochromatin and developmentally regulated gene clusters.
This recombinant rabbit monoclonal clone RM151 antibody is uniquely positioned for studies of stable but reversible chromatin repression. H3K9 dimethylation plays a critical role in lineage commitment, cellular differentiation, and maintenance of cell identity by ensuring selective silencing of gene programs.
At the molecular level, H3K9me2 is catalyzed by histone methyltransferases such as G9a and GLP and contributes to recruitment of chromatin-associated proteins including HP1 family members. These interactions promote chromatin compaction and establishment of repressive domains while allowing regulated transitions when required.
This modification is frequently associated with large chromatin blocks and coordinated gene silencing programs. Its presence reflects sustained repression rather than transient or intermediate chromatin states.
In western blot applications, the antibody detects Histone H3 at approximately 15 kDa, with signal corresponding to dimethylated chromatin associated with repressive domains. Detection reflects stable heterochromatin formation rather than transitional repression or active transcription.
At the cellular level, H3K9 dimethylation localizes to the nucleus and is enriched in regions of facultative heterochromatin. This distinguishes it from acetylation marks linked to activation and from monomethylated states associated with chromatin transition.
This antibody supports detection of Lys9-dimethylated Histone H3, enabling investigation of stable chromatin repression, heterochromatin formation, and epigenetic regulation of gene silencing.
The stated application concentrations are suggested starting points. Titration of the H3K9me2 Antibody / HIST1H3A Stable Repressive Chromatin Antibody may be required due to differences in protocols and secondary/substrate sensitivity.
A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys9) was used as the immunogen for this H3K9me2 Antibody / HIST1H3A Stable Repressive Chromatin Antibody.
Store the recombinant H3K9me2 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
Histone H3 Lys9 dimethylation antibody, H3K9me2 heterochromatin antibody, histone H3 di methyl Lys9 antibody, H3K9 dimethyl histone antibody
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