H3K79me2 Antibody / HIST1H3A Transcriptional Elongation Domain Antibody [clone RM181] (R20220)

Description

Histone H3 (HIST1H3A) methylation at lysine 79 is a unique chromatin modification located within the globular domain of histone H3, directly influencing nucleosome structure and transcriptional regulation. Dimethylation at lysine 79 represents a key marker of transcriptional elongation and gene body-associated chromatin. H3K79me2 Antibody / HIST1H3A Transcriptional Elongation Domain Antibody (clone RM181) is designed to detect Histone H3 dimethylated at lysine 79, providing a robust marker of actively transcribed chromatin regions. This antibody is part of a broader collection of Histone H3 antibodies used to study chromatin structure, histone modifications, and epigenetic regulation.

HIST1H3A antibody, also referred to as Histone H3 antibody and H3K79me2 antibody in the literature, recognizes a modification catalyzed exclusively by the DOT1L methyltransferase. Unlike tail-based histone modifications, lysine 79 methylation occurs within the nucleosome core, giving it a distinct structural and functional role in chromatin biology.

This recombinant rabbit monoclonal clone RM181 antibody is uniquely positioned for studies of transcriptional elongation and gene body chromatin organization. H3K79 dimethylation is enriched across actively transcribed genes and correlates with RNA polymerase II occupancy and elongation efficiency.

At the molecular level, H3K79me2 contributes to chromatin environments that support transcriptional elongation and stable gene expression. It may influence nucleosome stability and accessibility, facilitating efficient transcription through gene bodies.

This modification is strongly associated with active gene expression and is frequently used as a marker of transcriptionally engaged chromatin. Its distribution across coding regions provides a comprehensive view of elongation-associated chromatin states.

H3K79 methylation is also implicated in disease biology, particularly in leukemias driven by aberrant DOT1L activity, highlighting its importance in epigenetic regulation and transcriptional control.

In western blot applications, the antibody detects Histone H3 at approximately 15 kDa, with signal corresponding to dimethylated chromatin associated with transcriptionally active gene bodies. Detection reflects elongation-associated chromatin rather than promoter activation or repressive domains.

At the cellular level, H3K79 dimethylation localizes to the nucleus and is enriched in euchromatic regions undergoing active transcription. This distinguishes it from repressive marks such as H3K9me2 and H3K9me3.

This antibody supports detection of Lys79-dimethylated Histone H3, enabling investigation of transcriptional elongation, gene body chromatin organization, and epigenetic regulation of gene expression.

Application Notes

The stated application concentrations are suggested starting points. Titration of the H3K79me2 Antibody / HIST1H3A Transcriptional Elongation Domain Antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Immunogen

A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys79) was used as the immunogen for this H3K79me2 Antibody / HIST1H3A Transcriptional Elongation Domain Antibody.

Storage

Store the recombinant H3K79me2 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Alternate Names

Histone H3 Lys79 dimethylation antibody, H3K79me2 elongation antibody, histone H3 di methyl Lys79 antibody, H3K79 dimethyl histone antibody