- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
H3K79me1 Antibody / HIST1H3A Transcription-Linked Gene Body Methylation Antibody [clone RM147] (R20207)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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R20207-100UG | 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide | 100 ug | 485 | |
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R20207-25UG | 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide | 25 ug | 259 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Clone Name | RM147 |
| Purity | Protein A purified from animal origin-free supernatant |
| UniProt | P84243 |
| Gene ID | 8350 |
| Applications | Western Blot : 0.2-1ug/ml ChIP : 2-10ug/mg of lysate ELISA : 0.2-1ug/ml |
| Limitations | This recombinant H3K79me1 antibody is available for research use only. |
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Histone H3 (HIST1H3A) methylation at lysine 79 represents a distinctive epigenetic modification associated with gene body chromatin and transcription-linked regulatory processes. H3K79me1 Antibody / HIST1H3A Transcription-Linked Gene Body Methylation Antibody (clone RM147) is designed to detect Histone H3 monomethylated at lysine 79, providing a marker of chromatin states associated with active or permissive transcriptional regions. This antibody is part of a broader collection of Histone H3 antibodies used to study chromatin structure, histone modifications, and epigenetic regulation.
HIST1H3A antibody, also referred to as Histone H3 antibody and H3K79me1 antibody in the literature, recognizes a methylation site located within the globular core domain of Histone H3 rather than the N-terminal tail. This intranucleosomal positioning distinguishes Lys79 methylation from tail-based modifications such as H3K4me1 or H3K9me1 and contributes to its unique functional role in regulating chromatin structure within gene bodies.
This recombinant rabbit monoclonal clone RM147 antibody is uniquely positioned for studies of transcription-linked gene body methylation rather than enhancer priming or promoter activation. Compared with acetylation-based gene body marks such as H3K36ac or H3K79ac, H3K79me1 provides a methylation-based perspective on chromatin states associated with transcriptional continuity and chromatin organization across coding regions.
At the molecular level, H3K79 monomethylation is deposited by the methyltransferase DOT1L and is associated with transcriptionally active chromatin. It contributes to chromatin environments that support gene expression while maintaining structural integrity of nucleosomes during transcription.
H3K79 methylation exists in mono-, di-, and tri-methyl forms, each associated with different regulatory outcomes. The monomethylated state reflects an intermediate chromatin configuration that supports transcription without the stronger activation or specialization associated with higher methylation states.
In western blot applications, the antibody detects Histone H3 at approximately 15 kDa, with signal corresponding to monomethylated chromatin within transcription-associated regions. Detection reflects gene body methylation rather than enhancer activation or promoter-proximal chromatin states.
At the cellular level, H3K79 monomethylation localizes to the nucleus and is enriched in euchromatic domains corresponding to expressed genes. This distribution supports its use in studying transcription-linked chromatin regulation and gene body methylation architecture.
This antibody supports detection of Lys79-monomethylated Histone H3, enabling investigation of DOT1L-mediated methylation, transcription-associated chromatin regulation, and epigenetic control of gene body structure.
The stated application concentrations are suggested starting points. Titration of the H3K79me1 Antibody / HIST1H3A Transcription-Linked Gene Body Methylation Antibody may be required due to differences in protocols and secondary/substrate sensitivity.
A monomethyl-peptide corresponding to Monomethyl-Histone H3 (Lys79) was used as the immunogen for this H3K79me1 Antibody / HIST1H3A Transcription-Linked Gene Body Methylation Antibody.
Store the recombinant H3K79me1 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
Histone H3 Lys79 monomethylation antibody, H3K79me1 gene body antibody, histone H3 mono methyl Lys79 antibody, transcription-linked chromatin antibody
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