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Home >> Antibodies >> Recombinant H3K79me1 Antibody

Recombinant H3K79me1 Antibody [clone RM147] (R20207)

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  Catalog No Formulation Size Price (USD)  
Image R20207-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 469
Image
R20207-25UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 25 ug 239
Microvalidated Recrabbitmono
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Western blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2) using recombinant H3K79me1 antibody at 0.5 ug/ml showed a band of Histone H3 monomethylated at Lysine 79 (K79me1) in HeLa cells.
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Western blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2) using recombinant H3K79me1 antibody at 0.5 ug/ml showed a band of Histone H3 monomethylated at Lysine 79 (K79me1) in HeLa cells.
ChIP performed on HeLa cells using recombinant H3K79me1 antibody (5ug). Real-time PCR was performed using primers specific to the gene indicated.
The recombinant H3K79me1 antibody specifically reacts to Histone H3 monomethylated at Lysine 79 (K79me1). No cross reactivity with dimethylated (K79me2), trimethylated (K79me3), or other methylations in histone H3.
Availability 1-3 business days
Species Reactivity All Species
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM147
Purity Protein A purified from animal origin-free supernatant
UniProt P84243
Gene ID 8350
Applications Western blot : 0.2-1ug/ml
ChIP : 2-10ug/mg of lysate
ELISA : 0.2-1ug/ml
Limitations This recombinant H3K79me1 antibody is available for research use only.
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Description

This recombinant H3K79me1 antibody reacts to Histone H3 monomethylated at Lysine 79 (K79me1). No cross reactivity with dimethylated (K79me2), trimethylated (K79me3) or other methylations of Histone H3.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant H3K79me1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A monomethyl-peptide corresponding to Monomethyl-Histone H3 (Lys79) was used as the immunogen for this recombinant H3K79me1 antibody.

Storage

Store the recombinant H3K79me1 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Related Products

   1.   View all Recombinant antibodies

   2.   View all H3K79me1 antibodies

   3.   View all Histone H3 antibodies

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