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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
H3K4me2 Antibody / HIST1H3A Promoter Activation Readiness Antibody [clone RM135] (R20201)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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R20201-100UG | 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide | 100 ug | 485 | |
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R20201-25UG | 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide | 25 ug | 259 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Clone Name | RM135 |
| Purity | Protein A purified from animal origin-free supernatant |
| UniProt | P84243 |
| Gene ID | 8350 |
| Applications | Western Blot : 0.02ug/ml-0.2ug/ml Immunocytochemistry : 1-2ug/ml ELISA : 0.2-1ug/ml |
| Limitations | This recombinant H3K4me2 antibody is available for research use only. |
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Histone H3 (HIST1H3A) methylation at lysine 4 is a central feature of promoter-associated chromatin states and transcriptional initiation. Dimethylation at lysine 4 represents a chromatin configuration associated with promoter readiness and transcriptional activation potential. H3K4me2 Antibody / HIST1H3A Promoter Activation Readiness Antibody (clone RM135) is designed to detect Histone H3 dimethylated at lysine 4, providing a marker of promoters poised for transcriptional engagement. This antibody is part of a broader collection of Histone H3 antibodies used to study chromatin structure, histone modifications, and epigenetic regulation.
HIST1H3A antibody, also referred to as Histone H3 antibody and H3K4me2 antibody in the literature, recognizes a modification enriched at promoter regions and transcription start sites. This distinguishes H3K4me2 from H3K4me1, which marks enhancers, and H3K4me3, which is tightly associated with actively transcribing promoters. H3K4me2 occupies an intermediate activation state that reflects transcriptional competence without full transcriptional output.
This recombinant rabbit monoclonal clone RM135 antibody is uniquely positioned for studies of promoter readiness and transcription initiation. H3K4 dimethylation is commonly found at promoters that are accessible and prepared for activation but not yet fully engaged in high transcriptional activity.
At the molecular level, H3K4me2 contributes to recruitment of transcription factors and chromatin remodeling complexes that facilitate gene activation. It helps establish promoter architecture and maintain open chromatin conducive to transcription initiation.
This modification provides insight into promoter regions that are responsive to regulatory signals and capable of rapid activation. It represents a key step in the transition from inactive to active transcriptional states.
H3K4 methylation states form a gradient of promoter activity, with monomethylation marking enhancers, dimethylation indicating readiness, and trimethylation marking active transcription start sites.
In western blot applications, the antibody detects Histone H3 at approximately 15 kDa, with signal corresponding to dimethylated chromatin at promoter regions. Detection reflects transcription initiation potential rather than elongation or enhancer function.
At the cellular level, H3K4 dimethylation localizes to the nucleus and is enriched at promoter-associated chromatin. This supports its use in studying transcriptional activation readiness and promoter regulation.
This antibody supports detection of Lys4-dimethylated Histone H3, enabling investigation of promoter activation, transcription initiation, and epigenetic regulation of gene expression.
The stated application concentrations are suggested starting points. Titration of the H3K4me2 Antibody / HIST1H3A Promoter Activation Readiness Antibody may be required due to differences in protocols and secondary/substrate sensitivity.
A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys4) was used as the immunogen for this H3K4me2 Antibody / HIST1H3A Promoter Activation Readiness Antibody.
Store the recombinant H3K4me2 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
Histone H3 Lys4 dimethylation antibody, H3K4me2 promoter antibody, histone H3 di methyl Lys4 antibody, H3K4 dimethyl histone antibody
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