• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Order
Home >> Antibodies >> Recombinant H3K4me1 Antibody

Recombinant H3K4me1 Antibody [clone RM140] (R20200)

NSJ bio Image Pdf Image
  Catalog No Formulation Size Price (USD)  
Image R20200-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 469
Image
R20200-25UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 25 ug 239
Microvalidated Recrabbitmono
Bulk quote request
Western blot of recombinant histone H3.3 (1) and acid extracts of human HeLa cells (2) using the recombinant H3K4me1 antibody at 0.5 ug/ml showed a band of histone H3 monomethylated at Lysine 4 (K4me1).
›
Western blot of recombinant histone H3.3 (1) and acid extracts of human HeLa cells (2) using the recombinant H3K4me1 antibody at 0.5 ug/ml showed a band of histone H3 monomethylated at Lysine 4 (K4me1).
ICC testing of human HeLa cells treated with sodium butyrate using recombinant H3K4me1 antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
ChIP performed on human HeLa cells using 5ug recombinant H3K4me1 antibody. Real-time PCR was performed using primers specific to the gene indicated.
Recombinant H3K4me1 antibody specifically reacts to Histone H3 monomethylated at Lysine 4 (K4me1). No cross reactivity with dimethylated (K4me2), trimethylated (K4me3), or other methylations in histone H3.
Availability 1-3 business days
Species Reactivity All Species
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM140
Purity Protein A purified from animal origin-free supernatant
UniProt P84243
Gene ID 8350
Applications Western blot : 0.2-1ug/ml
ChIP : 2-10ug/mg of lysate
Immunocytochemistry : 1-2ug/ml
ELISA : 0.2-1ug/ml
Limitations This recombinant H3K4me1 antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

Description

This recombinant H3K4me1 antibody reacts to Histone H3 monomethylated at Lysine 4 (K4me1). No cross reactivity with dimethylated Lysine 4 (K4me2) or trimethylated Lysine 4 (K4me3), or other methylations in histone H3

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant H3K4me1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A monomethyl-peptide corresponding to Monomethyl-Histone H3 (Lys4) was used as the immunogen for this recombinant H3K4me1 antibody.

Storage

Store the recombinant H3K4me1 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Related Products

   1.   View all Recombinant antibodies

   2.   View all H3K4me1 antibodies

   3.   View all Histone H3 antibodies

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.