- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
H3K4ac Antibody / HIST1H3A Promoter-Proximal Activation Antibody [clone RM149] (R20213)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
R20213-100UG | 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide | 100 ug | 485 | |
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R20213-25UG | 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide | 25 ug | 259 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Clone Name | RM149 |
| Purity | Protein A purified from animal origin-free supernatant |
| UniProt | P84243 |
| Gene ID | 8350 |
| Applications | Western Blot : 0.25-1ug/ml Immunocytochemistry : 0.5-2ug/ml ELISA : 0.2-1ug/ml |
| Limitations | This H3K4ac antibody is available for research use only. |
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Histone H3 (HIST1H3A) acetylation at lysine 4 represents a regulatory modification associated with promoter-proximal chromatin and early transcriptional activation states. H3K4ac Antibody / HIST1H3A Promoter-Proximal Activation Antibody (clone RM149) is designed to detect Histone H3 acetylated at lysine 4, providing insight into chromatin environments that support transcriptional competence at gene promoters. This antibody is part of a broader collection of Histone H3 antibodies used to study chromatin structure, histone modifications, and epigenetic regulation.
HIST1H3A antibody, also referred to as Histone H3 antibody and H3K4ac antibody in the literature, recognizes a modification located near transcription start sites that contributes to chromatin accessibility and recruitment of transcriptional machinery. H3K4 acetylation is often associated with active or poised promoters and plays a role in establishing permissive chromatin environments for gene activation.
This recombinant rabbit monoclonal clone RM149 antibody is uniquely positioned for studies of promoter-proximal chromatin regulation. While H3K9ac is strongly associated with active promoter transcription and H3K27ac marks enhancers, H3K4ac reflects early regulatory events at promoters and contributes to chromatin states that precede or accompany transcriptional initiation.
At the molecular level, H3K4 acetylation influences recruitment of chromatin remodeling complexes and transcriptional regulators that establish transcriptionally competent promoter regions. It often co-occurs with H3K4 methylation, a well-known promoter-associated modification, but represents a distinct regulatory layer that reflects dynamic chromatin remodeling.
This modification contributes to fine-tuning of promoter architecture and can be associated with genes that are poised for activation or undergoing early transcriptional engagement. It provides an additional level of resolution in distinguishing promoter states within active chromatin.
In western blot applications, the antibody detects Histone H3 at approximately 15 kDa, with signal corresponding to acetylated chromatin at promoter-proximal regions. Detection reflects early-stage regulatory chromatin rather than elongation or enhancer-driven activation.
At the cellular level, H3K4 acetylation localizes to the nucleus and is enriched in euchromatic regions corresponding to gene promoters. This distribution supports its use as a marker of promoter architecture and transcriptional regulation.
This antibody supports detection of Lys4-acetylated Histone H3, enabling investigation of promoter chromatin organization, transcriptional activation, and epigenetic regulation of gene expression.
The stated application concentrations are suggested starting points. Titration of the H3K4ac Antibody / HIST1H3A Promoter-Proximal Activation Antibody may be required due to differences in protocols and secondary/substrate sensitivity.
An acetyl-peptide corresponding to the Acetyl-Histone H3 (Lys4) was used as the immunogen for this H3K4ac Antibody / HIST1H3A Promoter-Proximal Activation Antibody.
Store the recombinant H3K4ac antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
Histone H3 Lys4 acetylation antibody, H3K4ac promoter antibody, histone H3 acetyl Lys4 antibody, promoter chromatin marker antibody
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