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Home >> Antibodies >> Recombinant H3K27ac Antibody

Recombinant H3K27ac Antibody [clone RM172] (R20210)

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  Catalog No Formulation Size Price (USD)  
Image R20210-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 485
Image
R20210-25UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 25 ug 249
Microvalidated Recrabbitmono
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Western blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate using recombinant H3K27ac antibody at 1 ug/ml showed a band of Histone H3 acetylated at Lysine 27 in treated HeLa cells.
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Western blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate using recombinant H3K27ac antibody at 1 ug/ml showed a band of Histone H3 acetylated at Lysine 27 in treated HeLa cells.
The recombinant H3K27ac antibody specifically reacts to Histone H3 acetylated at Lysine 27 (K27ac). No cross reactivity with acetylated Lysine 4 (K4ac), 9 (K9ac), 14 (K14ac), 18 (K18ac), 23 (K23ac), 36 (K36ac), 56 (K56ac), 79 (K79ac), or 122 (K122) in Histone H3.
ICC/IF test of HeLa cells treated with sodium butyrate using recombinant H3K27ac antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Availability 1-3 business days
Species Reactivity All Species
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM172
Purity Protein A purified from animal origin-free supernatant
UniProt P84243
Gene ID 8350
Applications Western Blot : 0.5-2ug/ml
Immunocytochemistry : 0.5-2ug/ml
ELISA : 0.2-1ug/ml
Limitations This recombinant H3K27ac antibody is available for research use only.
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Description

This recombinant H3K27ac antibody reacts to Histone H3 acetylated at Lysine 27 (K27ac). No cross reactivity with other acetylated Lysines in Histone H3.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant H3K27ac antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An acetyl-peptide corresponding to the Acetyl-Histone H3 (Lys27) was used as the immunogen for this recombinant H3K27ac antibody.

Storage

Store the recombinant H3K27ac antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Related Products

   1.   View all Recombinant antibodies

   2.   View all H3K27ac antibodies

   3.   View all Histone H3 antibodies

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