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Home >> Antibodies >> Recombinant H3K18ac Antibody

Recombinant H3K18ac Antibody [clone RM166] (R20209)

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  Catalog No Formulation Size Price (USD)  
Image R20209-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 485
Image
R20209-25UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 25 ug 249
Microvalidated Recrabbitmono
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Western blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate using recombinant H3K18ac antibody at 0.5 ug/mL showed a band of Histone H3 acetylated at Lysine 18 in treated HeLa cells.
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Western blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate using recombinant H3K18ac antibody at 0.5 ug/mL showed a band of Histone H3 acetylated at Lysine 18 in treated HeLa cells.
Sandwich ELISA with acetylated Histone H3 at Lys 18 using human HeLa cell lysate, treated or untreated with sodium butyrate, using anti-Histone H3CT (RM188, 1 ug/ml) as the capture and biotinylated recombinant H3K18ac antibody (RM166, 2 ug/ml) as the detect.
ChIP performed on HeLa cells with or without sodium butyrate treatment using recombinant H3K18ac antibody (5ug). Real-time PCR was performed using primers specific to the gene indicated.
The recombinant H3K18ac antibody specifically reacts to Histone H3 acetylated at Lysine 18 (K18ac). No cross reactivity with acetylated 4 (K4ac), 9 (K9ac), 14 (K14ac), 23 (K23ac), 27 (K27ac), 36 (K36ac), 56 (K56ac), 79 (K79ac), or 122 (K122) in Histone H3.
ICC/IF test of HeLa cells treated with sodium butyrate using recombinant H3K18ac antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
ICC staining of human HepG2 cells using recombinant H3K18ac antibody.
Availability 1-3 business days
Species Reactivity All Species
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM166
Purity Protein A purified from animal origin-free supernatant
UniProt P84243
Gene ID 8350
Applications Western Blot : 0.5-2ug/ml
Immunocytochemistry : 0.5-2ug/ml
ChIP : 2ug/ml-10ug/mg of lysate
Immunohistochemistry : 1-10ug/ml (1)
ELISA : 0.2-1ug/ml
Limitations This recombinant H3K18ac antibody is available for research use only.
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Description

This recombinant H3K18ac antibody reacts to Histone H3 acetylated at Lysine 18 (K18ac). No cross reactivity with other acetylated Lysines in histone H3.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant H3K18ac antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Immunogen

An acetyl-peptide corresponding to the Acetyl-Histone H3 (Lys18) was used as the immunogen for this recombinant H3K18ac antibody.

Storage

Store the recombinant H3K18ac antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Related Products

   1.   View all Recombinant antibodies

   2.   View all H3K18ac antibodies

   3.   View all Histone H3 antibodies

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