H3K18ac Antibody / HIST1H3A Transcription Activation Co-Activator Antibody [clone RM166] (R20209)

Description

Histone H3 (HIST1H3A) is a core nucleosomal protein that undergoes acetylation at specific lysine residues to regulate chromatin structure and transcriptional activity. Acetylation at lysine 18 is a key modification associated with transcriptional activation and co-activator-mediated gene regulation. H3K18ac Antibody / HIST1H3A Transcription Activation Co-Activator Antibody (clone RM166) is designed to detect Histone H3 acetylated at lysine 18, providing a marker of actively engaged transcriptional machinery. This antibody is part of a broader collection of Histone H3 antibodies used to study chromatin structure, histone modifications, and epigenetic regulation.

HIST1H3A antibody, also referred to as Histone H3 antibody and H3K18ac antibody in the literature, recognizes a modification frequently catalyzed by histone acetyltransferases such as p300 and CBP. These enzymes play central roles in transcriptional activation by remodeling chromatin and recruiting transcriptional co-activator complexes that facilitate assembly of RNA polymerase II and associated regulatory proteins.

This recombinant rabbit monoclonal clone RM166 antibody is uniquely positioned for studies of transcriptional activation driven by co-activator recruitment rather than chromatin accessibility alone. While marks such as H3K9ac and H3K14ac are associated with open chromatin, H3K18ac more directly reflects active engagement of transcriptional complexes and signal-dependent gene activation events.

At the molecular level, H3K18 acetylation promotes recruitment of bromodomain-containing proteins and transcriptional co-activators that stabilize transcriptional complexes at regulatory regions. It is often enriched at promoters and regulatory elements undergoing rapid activation in response to signaling pathways, including stress responses and developmental cues.

H3K18 acetylation frequently occurs in combination with other activating histone marks, forming part of a coordinated epigenetic signature that defines transcriptionally active chromatin. Its presence indicates not only chromatin accessibility but also active regulatory engagement and transcriptional output.

In western blot applications, the antibody detects Histone H3 at approximately 15 kDa, with signal corresponding to acetylated chromatin in transcriptionally active cells. Detection reflects co-activator-driven gene activation rather than structural chromatin changes alone.

At the cellular level, H3K18 acetylation localizes to the nucleus and is enriched in euchromatic regions associated with active transcription and regulatory signaling. This distinguishes it from elongation-associated and enhancer-specific acetylation marks.

This antibody supports detection of Lys18-acetylated Histone H3, enabling investigation of transcriptional activation, co-activator recruitment, and epigenetic regulation of gene expression.

Application Notes

The stated application concentrations are suggested starting points. Titration of the H3K18ac Antibody / HIST1H3A Transcription Activation Co-Activator Antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Immunogen

An acetyl-peptide corresponding to the Acetyl-Histone H3 (Lys18) was used as the immunogen for this H3K18ac Antibody / HIST1H3A Transcription Activation Co-Activator Antibody.

Storage

Store the recombinant H3K18ac antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Alternate Names

Histone H3 Lys18 acetylation antibody, H3K18ac activation antibody, histone H3 acetyl Lys18 antibody, transcription co-activator histone antibody