- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
H3K14ac Antibody / HIST1H3A Chromatin Accessibility Activation Antibody [clone RM130] (R20199)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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R20199-100UG | 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide | 100 ug | 485 | |
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R20199-25UG | 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide | 25 ug | 259 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Clone Name | RM130 |
| Purity | Protein A purified from animal origin-free supernatant |
| UniProt | P84243 |
| Gene ID | 8350 |
| Applications | Western Blot : 0.5-2ug/ml Immunocytochemistry : 0.5-2ug/ml ChIP : 2-10ug/mg of lysate ELISA : 0.2-1ug/ml |
| Limitations | This H3K14ac antibody is available for research use only. |
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Histone H3 (HIST1H3A) is a core nucleosomal protein that undergoes acetylation at specific lysine residues to regulate chromatin structure and gene expression. Acetylation at lysine 14 is a key modification associated with open chromatin architecture and transcriptionally permissive genomic regions. H3K14ac Antibody / HIST1H3A Chromatin Accessibility Activation Antibody (clone RM130) is designed to detect Histone H3 acetylated at lysine 14, providing a robust marker of chromatin accessibility and regulatory activation. This antibody is part of a broader collection of Histone H3 antibodies used to study chromatin structure, histone modifications, and epigenetic regulation.
HIST1H3A antibody, also referred to as Histone H3 antibody and H3K14ac antibody in the literature, recognizes a modification catalyzed by histone acetyltransferases that neutralizes the positive charge of lysine residues. This reduces histone-DNA affinity and promotes nucleosome relaxation, allowing increased access of transcription factors and chromatin remodeling complexes. H3K14 acetylation is enriched in euchromatic regions and is frequently associated with genes undergoing active transcription or regulatory activation.
This recombinant rabbit monoclonal clone RM130 antibody is uniquely positioned as a chromatin accessibility marker that complements but remains distinct from H3K9ac. While H3K9ac is strongly promoter-associated, H3K14ac is more broadly linked to global chromatin opening and regulatory region accessibility, making it particularly useful for identifying permissive chromatin environments across the genome.
Importantly, this antibody specifically detects Lys14-acetylated Histone H3 and is not affected by neighboring modifications. It shows no cross-reactivity with acetylation at Lys4, Lys9, Lys18, Lys23, Lys27, Lys36, Lys56, or Lys79, ensuring precise and site-specific detection of the K14ac modification in complex chromatin contexts.
At the molecular level, H3K14 acetylation facilitates recruitment of bromodomain-containing proteins, transcriptional co-activators, and ATP-dependent chromatin remodeling complexes. These interactions promote formation of accessible chromatin domains that support transcriptional activation and regulatory responsiveness.
In western blot applications, the antibody detects Histone H3 at approximately 15 kDa, with signal corresponding to acetylated chromatin associated with accessible genomic regions. Detection reflects chromatin accessibility rather than strictly promoter activity or transcriptional elongation.
At the cellular level, H3K14 acetylation localizes to the nucleus and is enriched in euchromatic regions characterized by open chromatin and active regulatory potential. This distribution distinguishes it from repressive histone marks and mitosis-associated modifications.
This antibody supports detection of Lys14-acetylated Histone H3, enabling detailed investigation of chromatin accessibility, regulatory region activation, and epigenetic control of gene expression.
The stated application concentrations are suggested starting points. Titration of the H3K14ac Antibody / HIST1H3A Chromatin Accessibility Activation Antibody may be required due to differences in protocols and secondary/substrate sensitivity.
An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys14) was used as the immunogen for this H3K14ac Antibody / HIST1H3A Chromatin Accessibility Activation Antibody.
Store the recombinant H3K14ac antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
Histone H3 Lys14 acetylation antibody, H3K14ac chromatin activation antibody, histone H3 acetyl Lys14 antibody, open chromatin histone antibody
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