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Home >> Antibodies >> Recombinant H2BK23ac Antibody

Recombinant H2BK23ac Antibody [clone RM260] (R20242)

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  Catalog No Formulation Size Price (USD)  
Image R20242-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 485
Image
R20242-25UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 25 ug 249
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Western blot test of (1) acid extracts of HeLa cells treated with sodium butyrate, (2) acid extracts of HeLa cells untreated, and (3) recombinant Histone H2B, using recombinant H2BK23ac antibody at 0.5 ug/ml.
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Western blot test of (1) acid extracts of HeLa cells treated with sodium butyrate, (2) acid extracts of HeLa cells untreated, and (3) recombinant Histone H2B, using recombinant H2BK23ac antibody at 0.5 ug/ml.
This recombinant H2BK23ac antibody specifically reacts to Histone H2B acetylated at Lysine 23 (K23ac). No cross reactivity with acetylated Lysine 5 (K5ac), 11 (K11ac), 12 (K12ac), 15 (K15ac), 20 (K20ac), or non-modified Lysine 23 in Histone H2B.
ICC/IF of HeLa cells treated with sodium butyrate, using recombinant H2BK23ac antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM260
Purity Protein A purified from animal origin-free supernatant
UniProt P33778
Gene ID 3018
Applications Western Blot : 0.5-2ug/ml
Immunocytochemistry : 0.5-2ug/ml
ELISA : 0.2-1ug/ml
Limitations This recombinant H2BK23ac antibody is available for research use only.
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Description

This recombinant H2BK23ac antibody reacts to Histone H2B acetylated at Lysine 23 (K23ac). No cross reactivity with other acetylated Lysines in histones.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant H2BK23ac antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An acetyl-peptide corresponding to Acetyl-Histone H2B (Lys23) was used as the immunogen for this recombinant H2BK23ac antibody.

Storage

Store the recombinant H2BK23ac antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Related Products

   1.   View all Recombinant antibodies

   2.   View all H2BK23ac antibodies

   3.   View all Histone H2B antibodies

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