Recombinant GPC3 Antibody / Glypican-3 [clone rGPC3/863] (V3714)

IHC testing of FFPE human hepatocellular carcinoma with recombinant GPC3 antibody (clone rGPC3/863). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.

Immunofluorescent staining of methanol-fixed HepG2 cells with recombinant GPC3 antibody (green, clone rGPC3/863) and Reddot nuclear stain (red).

Flow cytometry testing of methanol-fixed human HepG2 cells with recombinant GPC3 antibody (clone rGPC3/863); Red=isotype control, Blue= recombinant GPC3 antibody.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using recombinant GPC3 antibody (clone rGPC3/863). These results demonstrate the foremost specificity of the rGPC3/863 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free recombinant GPC3 antibody (clone rGPC3/863) as confirmation of integrity and purity.