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Home >> Antibodies >> Recombinant GP2 Antibody / Glycoprotein 2 / ZAP75

Recombinant GP2 Antibody / Glycoprotein 2 / ZAP75 [clone rGP2/8617] (V4409)

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  Catalog No Formulation Size Price (USD)  
Image V4409-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4409-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4409SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human pancreas tissue with recombinant GP2 antibody (clone rGP2/8617). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human pancreas tissue with recombinant GP2 antibody (clone rGP2/8617). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant GP2 antibody (clone rGP2/8617) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rGP2/8617
Purity Protein A/G affinity
UniProt P55259
Localization Cell surface, Secreted, Cytoplasmic
Applications ELISA : 2-4mg/ml for coating (order BSA-free format)
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This recombinant GP2 antibody is available for research use only.
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Description

GP2 (glycoprotein 2), also known as ZAP75, is a 537 amino acid secreted protein. It is an integral membrane protein that is secreted from intracellular zymogen granules and associates with the plasma membrane via glycosylphosphatidylinositol (GPI) linkage. GP2 is cleaved and then released into the pancreatic duct along with exocrine secretions. GP2 binds pathogens such as enterobacteria, thereby playing an important role in the innate immune response. The C-terminus of this protein is related to the C-terminus of the protein encoded by the neighboring gene, uromodulin (UMOD). GP2 is also expressed on the apical plasma membrane of specialized microfold (M) cells among enterocytes and serves as a transcytotic receptor for mucosal antigens. M cells are considered a promising target for oral vaccination against various infectious diseases.

Application Notes

Optimal dilution of the recombinant GP2 antibody should be determined by the researcher.

Immunogen

A recombinant fragment of human protein (within amino acids 35-179) was used as the immunogen for the recombinant GP2 antibody.

Storage

Aliquot the recombinant GP2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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