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Home >> Antibodies >> Recombinant Golgi Antibody

Recombinant Golgi Antibody [clone GLG1/2829R] (V7503)

  Catalog No Formulation Size Price (USD)  
Image V7503-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7503-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7503SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V7503IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human breast carcinoma with recombinant Golgi antibody (clone GLG1/2829R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant Golgi antibody (clone GLG1/2829R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name GLG1/2829R
Purity Protein A affinity chromatography
UniProt Q92896
Localization Golgi complex in cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This recombinant Golgi antibody is available for research use only.
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Description

This MAb recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae.

Application Notes

The optimal dilution of the recombinant Golgi antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Full length recombinant GLG1 protein was used as the immunogen for this recombinant Golgi antibody.

Storage

Store the recombinant Golgi antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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