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Home >> Antibodies >> Recombinant Glycophorin A Antibody / GYPA / Rabbit Monoclonal

Recombinant Glycophorin A Antibody / GYPA / Rabbit Monoclonal [clone SGPA35-2R] (V3725)

  Catalog No Formulation Size Price (USD)  
Image V3725-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3725-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3725SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V3725IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human angiosarcoma with recombinant Glycophorin A antibody (clone SGPA35-2R). Required HIER: steam section in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool prior to staining.
Western blot testing of human K562 cell lysate with Glycophorin A antibody (clone SGPA35-2R). Expected molecular weight: routinely observed at ~16/38 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name SGPA35-2R
Purity Protein A affinity chromatography
UniProt P02724
Localization Cytoplasmic, membranous
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 0.25-0.5ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This recombinant Glycophorin A antibody is available for research use only.
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Description

Glycophorin A (MNS blood group), also known as GYPA, is a protein which in humans is encoded by the GYPA gene. GYPA has also been designated CD235a. Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. [Wiki]

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Glycophorin A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human GYPA protein was used as the immunogen for the recombinant Glycophorin A antibody.

Storage

Store the recombinant Glycophorin A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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