GLUT1 Antibody Recombinant Rabbit MAb GLUT1/3132R / SLC2A1 Glucose Transporter Antibody [clone GLUT1/3132R] (V7457)

GLUT1 Antibody Recombinant Rabbit MAb GLUT1/3132R. Immunohistochemistry analysis of GLUT1 / SLC2A1 in FFPE human tongue tissue shows strong HRP-DAB brown membranous staining in epithelial cells, consistent with the plasma membrane localization of the GLUT1 glucose transporter. The staining outlines the borders of stratified epithelial cells and highlights the characteristic membrane-associated distribution of GLUT1 in metabolically active epithelial tissue. Stromal and connective tissue components show minimal staining, providing contrast to the epithelial signal. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris buffer with 1mM EDTA for 10-20 minutes followed by cooling at room temperature prior to antibody incubation.

GLUT1 Antibody Recombinant Rabbit MAb GLUT1/3132R. Flow cytometry analysis of human K562 cells stained with recombinant rabbit monoclonal GLUT1 antibody demonstrates a clear rightward shift of the fluorescence signal (blue histogram) compared with the isotype control (red histogram), indicating specific detection of the GLUT1 / SLC2A1 glucose transporter on the analyzed cell population. The fluorescence intensity distribution shows a distinct population of GLUT1-positive cells consistent with surface-associated expression of this facilitative glucose transporter in metabolically active leukemia cells. Data are presented as FITC-A fluorescence intensity normalized to mode, with the recombinant GLUT1 antibody producing strong target-specific staining relative to the control antibody.

SDS-PAGE analysis of purified, BSA-free recombinant GLUT1 antibody GLUT1/3132R as confirmation of integrity and purity.