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Home >> Antibodies >> Recombinant GLUT1 Antibody

Recombinant GLUT1 Antibody [clone GLUT1/3132R] (V7457)

  Catalog No Formulation Size Price (USD)  
Image V7457-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7457-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7457SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V7457-IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Microvalidated Recrabbitmono
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IHC testing of human tongue with recombinant GLUT1 antibody. Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Flow cytometry testing of human K562 cells with recombinant GLUT1 antibody (clone GLUT1/3132R); Red=isotype control, Blue= recombinant GLUT1 antibody.
SDS-PAGE analysis of purified, BSA-free recombinant GLUT1 antibody as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name GLUT1/3132R
Purity Protein G affinity chromatography
UniProt P11166
Localization Cell surface
Applications ELISA : 1-2ug/ml for coating (order BSA/sodium azide-free format)
Flow cytometry : 1-2ug/10^6 cells
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This recombinant GLUT1 antibody is available for research use only.
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Description

Recognizes a protein of 45-55kDa, which is identified as GLUT1. Glucose transporters are integral membrane glycoproteins involved in transporting glucose into most cells. There are many types of glucose transport carrier proteins, designated as Glut1 to Glut12. Glut1 is a major glucose transporter in the mammalian blood-brain barrier. It is expressed in high density on the membranes of human erythrocytes and the brain capillaries that comprise the blood-brain barrier. Glut1 is expressed at variable levels in many human tissues. Overexpression has been linked to tumor progression or poor survival of patients with carcinomas of the colon, breast, cervical, lung, bladder and mesothelioma. Glut1 is a sensitive and specific marker for the differentiation of malignant mesothelioma (positive) from reactive mesothelium (negative).

Application Notes

Optimal dilution of the recombinant GLUT1 antibody should be determined by the researcher.

Immunogen

A portion of amino acids 203-305 from the human protein was used as the immunogen for this recombinant GLUT1 antibody.

Storage

Store the recombinant GLUT1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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