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Home >> Antibodies >> Recombinant GFAP Antibody / Rabbit Monoclonal

Recombinant GFAP Antibody / Rabbit Monoclonal [clone ASTRO/1974R] (V3588)

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  Catalog No Formulation Size Price (USD)  
Image V3588-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3588-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3588SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V3588IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Microvalidated Recrabbitmono
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Immunofluorescent staining of frozen human cerebral cortex tissue with recombinant GFAP antibody (blue, clone ASTRO/1974R) and Histone H1 antibody (magenta, clone HH1/957).
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Immunofluorescent staining of frozen human cerebral cortex tissue with recombinant GFAP antibody (blue, clone ASTRO/1974R) and Histone H1 antibody (magenta, clone HH1/957).
IHC testing of FFPE human cerebellum tissue with recombinant GFAP antibody (clone ASTRO/1974R). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free recombinant GFAP antibody (clone ASTRO/1974R) as confirmation of integrity and purity.
Flow cytometry testing of fixed human T98G cells with recombinant GFAP antibody (clone ASTRO/1974R); Red=isotype control, Blue= recombinant GFAP antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name ASTRO/1974R
Purity Protein A affinity chromatography
UniProt P14136
Localization Cytoplasmic
Applications Flow cytometry : 1-2ug/10^6 cells
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Immunofluorescence : 1-2ug/ml
Limitations This recombinant GFAP antibody is available for research use only.
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Description

This mAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Application Notes

The optimal dilution of the recombinant GFAP antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

GFAP isolated from pig spinal cord was used as the immunogen for this recombinant GFAP antibody.

Storage

Store the recombinant GFAP antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Related Products

   1.   View all GFAP antibodies

   2.   View all recombinant antibodies

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