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Home >> Antibodies >> Recombinant GFAP Antibody / Glial Fibrillary Acidic Protein

Recombinant GFAP Antibody / Glial Fibrillary Acidic Protein [clone rGFAP/9150] (V5483)

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  Catalog No Formulation Size Price (USD)  
Image V5483-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 539
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V5483-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 249
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V5483SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
Microvalidated Recmousemono
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Western blot testing of human brain tissue lysate with recombinant GFAP antibody (clone rGFAP/9150). Predicted molecular weight ~50 kDa.
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Western blot testing of human brain tissue lysate with recombinant GFAP antibody (clone rGFAP/9150). Predicted molecular weight ~50 kDa.
IHC staining of FFPE human brain tissue with recombinant GFAP antibody (clone rGFAP/9150). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant GFAP antibody (clone rGFAP/9150) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG2b, kappa
Clone Name rGFAP/9150
Purity Protein A/G affinity
UniProt P14136
Localization Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Western Blot : 2-4ug/ml
Limitations This recombinant GFAP antibody is available for research use only.
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Description

This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Application Notes

Optimal dilution of the recombinant GFAP antibody should be determined by the researcher.

Immunogen

A recombinant fragment (within amino acids 100-300) of human Glial Fibrillary Acidic Protein protein was used as the immunogen for the recombinant GFAP antibody.

Storage

Aliquot the recombinant GFAP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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