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Home >> Antibodies >> GCSF Antibody / Granulocyte-Colony Stimulating Factor

GCSF Antibody / Granulocyte-Colony Stimulating Factor [clone CSF3/3166R] (V7752)

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  Catalog No Formulation Size Price (USD)  
Image V7752-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 559
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V7752-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 259
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V7752SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
Microvalidated Recrabbitmono
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GCSF Antibody Human Tonsil Tissue Immunohistochemistry. IHC staining of FFPE human tonsil with GCSF antibody (clone CSF3/3166R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant GCSF antibody (clone CSF3/3166R) as confirmation of integrity and purity.
CSF3 Antibody Human Liver WB. Western blot analysis of Granulocyte colony stimulating factor / CSF3 in human liver tissue lysates using rabbit monoclonal CSF3 antibody, clone CSF3/3166R. Two lanes are shown at different antibody dilutions (1:100 and 1:50), with bands detected at approximately 18-20 kDa, consistent with the predicted molecular weight of G-CSF, supporting detection of this hematopoietic cytokine in liver tissue.
CSF3 Antibody Liver Tissue WB. Western blot analysis of Granulocyte colony stimulating factor / CSF3 in human liver tissue lysate using rabbit monoclonal CSF3 antibody, clone CSF3/3166R. A band is detected at approximately 18-20 kDa, consistent with the predicted molecular weight of G-CSF, supporting detection of this hematopoietic cytokine in liver tissue.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name CSF3/3166R
Purity Protein A affinity chromatography
UniProt P09919
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Western Blot : 2-4ug/ml
Limitations This GCSF antibody is available for research use only.
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Description

GCSF Antibody recognizes granulocyte-colony stimulating factor (G-CSF) in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell types. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. It reacts with early precursor and mature forms of myeloid cells. It is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes. G-CSF is a pleiotropic cytokine that influences differentiation, proliferation and activation of the neutrophilic granulocyte lineage. The human G-CSF cDNA encodes a 207 amino acid precursor containing a 29 amino acid signal peptide that is proteolytically cleaved to form a 178 amino acid residue mature protein. Two G-CSF s, which are identical except for a three amino acid deletion in the amino-terminus of one form of the protein have been isolated from human cells.

For a reference GCSF antibody supporting cytokine signaling and immune regulation studies, see clone CSF3/4594.

Application Notes

Optimal dilution of the GCSF antibody should be determined by the researcher.

Immunogen

A recombinant full-length human CSF3 protein was used as the immunogen for the recombinant GCSF antibody.

Storage

Store the GCSF antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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