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Home >> Antibodies >> Recombinant GCSF Antibody / Granulocyte-Colony Stimulating Factor

Recombinant GCSF Antibody / Granulocyte-Colony Stimulating Factor [clone CSF3/3166R] (V7752)

  Catalog No Formulation Size Price (USD)  
Image V7752-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7752-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7752SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human tonsil with recombinant GCSF antibody (clone CSF3/3166R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant GCSF antibody (clone CSF3/3166R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name CSF3/3166R
Purity Protein A affinity chromatography
UniProt P09919
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant GCSF antibody is available for research use only.
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Description

This MAb recognizes granulocyte-colony stimulating factor (G-CSF) in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell types. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. It reacts with early precursor and mature forms of myeloid cells. It is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes. G-CSF is a pleiotropic cytokine that influences differentiation, proliferation and activation of the neutrophilic granulocyte lineage. The human G-CSF cDNA encodes a 207 amino acid precursor containing a 29 amino acid signal peptide that is proteolytically cleaved to form a 178 amino acid residue mature protein. Two G-CSF s, which are identical except for a three amino acid deletion in the amino-terminus of one form of the protein have been isolated from human cells.

Application Notes

Optimal dilution of the recombinant GCSF antibody should be determined by the researcher.

Immunogen

A recombinant full-length human CSF3 protein was used as the immunogen for the recombinant GCSF antibody.

Storage

Store the recombinant GCSF antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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