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Home >> Antibodies >> Recombinant GATA-binding factor 3 Antibody / GATA3

Recombinant GATA-binding factor 3 Antibody / GATA3 [clone rGATA3/9133] (V5520)

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  Catalog No Formulation Size Price (USD)  
Image V5520-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 539
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V5520-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 249
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V5520SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
Microvalidated Recmousemono
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IHC staining of FFPE human breast tissue with recombinant GATA-binding factor 3 antibody (clone rGATA3/9133). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human breast tissue with recombinant GATA-binding factor 3 antibody (clone rGATA3/9133). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant GATA-binding factor 3 antibody (clone rGATA3/9133) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG2b, kappa
Clone Name rGATA3/9133
Purity Protein A/G affinity
UniProt P23771
Localization Nucleus
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant GATA-binding factor 3 antibody is available for research use only.
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Description

GATA-3 (GATA binding protein 3) is a member of the GATA family of transcription factors. This 50kDa nuclear protein regulates the development and subsequent maintenance of a variety of human tissues, including hematopoietic cells, skin, kidney, mammary gland, and the central nervous system. Among several other roles, GATA-3 involved in luminal cell differentiation in the mammary gland and appears to control a set of genes involved in the differentiation and proliferation of breast cancer. The expression of GATA-3 is associated with the expression of estrogen receptor-alpha (ER) in breast cancer. GATA-3 has been shown to be a novel marker for bladder cancer. The study demonstrated that GATA-3 stained 67% of urothelial Carcinomas, but none of prostate or renal carcinomas.

Application Notes

Optimal dilution of the recombinant GATA-binding factor 3 antibody should be determined by the researcher.

Immunogen

A recombinant fragment (within amino acids 200-300) of human GATA-3 protein was used as the immunogen for the recombinant GATA-binding factor 3 antibody.

Storage

Aliquot the recombinant GATA-binding factor 3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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