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Home >> Antibodies >> Recombinant GAPDH Antibody (Loading Control)

Recombinant GAPDH Antibody (Loading Control) [clone RM114] (R20198)

  Catalog No Formulation Size Price (USD)  
Image R20198-0.1ML Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ul 379
Western blot of human A431 cell lysate using the recombinant GAPDH antibody at 1:1000. Predicted molecular weight ~36 kDa.
ICC staining of human HeLa cells using recombinant GAPDH antibody at 1:200 (red) and DAPI nuclear stain (blue).
Availability 1-3 business days
Species Reactivity All Species
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM114
Purity Protein A purified from animal origin-free supernatant
UniProt P04406
Gene ID 2597
Applications Western blot : 1:1000
Immunoprecipitation : 1:200
Chromatin IP (ChIP) : 1:200
Immunocytochemistry : 1:200
Immunohistochemistry : 1:200 (1)
Limitations This recombinant GAPDH antibody is available for research use only.
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Description

This recombinant GAPDH antibody reacts to Glyceraldehyde 3-phosphate dehydrogenase.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant GAPDH antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Immunogen

A peptide corresponding to the C-terminus of GAPDH was used as the immunogen for this recombinant GAPDH antibody.

Storage

Store the recombinant GAPDH antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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