LGALS1 Antibody / Glycan Binding Lectin Antibody [clone GAL1/2499R] (V7302)

LGALS1 Antibody HeLa WB. Western blot analysis of human HeLa cell lysate using recombinant LGALS1 antibody (clone GAL1/2499R). A strong, distinct immunoreactive band is detected at approximately 14 kDa, corresponding to the expected molecular weight of LGALS1, also known as Galectin-1. LGALS1 is a beta-galactoside-binding lectin that regulates cell adhesion, immune homeostasis, apoptosis, angiogenesis, and glycan-mediated signaling pathways. The clean single-band staining pattern supports the specificity of recombinant clone GAL1/2499R for detection of endogenous LGALS1 protein by western blot. Predicted molecular weight: ~14 kDa.

LGALS1 Antibody JEG-3 and K562 WB. Western blot analysis of 1) human JEG-3 and 2) human K562 cell lysates using recombinant LGALS1 antibody (clone GAL1/2499R). A prominent immunoreactive band is detected at approximately 14 kDa in both cell lines, consistent with the expected molecular weight of LGALS1. LGALS1 is a beta-galactoside-binding lectin involved in glycan-mediated signaling, immune regulation, cell adhesion, angiogenesis, and tumor microenvironment remodeling. The strong and specific detection of endogenous LGALS1 in multiple human cell lines demonstrates the utility of recombinant clone GAL1/2499R for western blot applications. Predicted molecular weight: ~14 kDa.

LGALS1 Antibody Prostate Carcinoma IHC. Immunohistochemical staining of FFPE human prostate carcinoma tissue using recombinant LGALS1 antibody (clone GAL1/2499R) demonstrates prominent cytoplasmic and stromal-associated HRP-DAB brown staining within the tumor microenvironment. Strong immunoreactivity is observed in fibroblast-like stromal cells and connective tissue structures surrounding malignant glandular elements, consistent with the known expression pattern of LGALS1, a beta-galactoside-binding lectin involved in immune regulation, extracellular matrix remodeling, angiogenesis, and tumor progression. The staining pattern highlights the important role of LGALS1 in tumor-stromal interactions and supports the use of recombinant clone GAL1/2499R for detection of LGALS1 expression in FFPE tissue specimens. HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min and allow to cool before testing.

LGALS1 Antibody Protein Microarray Validation. Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using recombinant LGALS1 antibody (clone GAL1/2499R) demonstrates exceptional target specificity for LGALS1. The GAL1/2499R recombinant monoclonal antibody identifies LGALS1 as the highest-ranking target with a Z-score of 148.81 and an S-score of 134.45, substantially exceeding all non-target proteins represented on the array. These results confirm the foremost specificity of clone GAL1/2499R and provide strong evidence for highly selective recognition of LGALS1 with minimal off-target binding. Z-score represents the signal strength generated by antibody binding to a specific protein relative to all proteins present on the array, while S-score reflects the specificity gap between the intended target and the next highest-ranking protein. An S-score greater than 2.5 is generally considered indicative of highly specific target recognition. The exceptionally high Z- and S-scores observed for GAL1/2499R support its suitability for applications requiring accurate and reliable detection of endogenous LGALS1 expression.

SDS-PAGE analysis of purified, BSA-free recombinant Galectin 1 antibody (clone GAL1/2499R) as confirmation of integrity and purity.