- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
GAD2 Antibody Recombinant Mouse MAb / GAD65 [clone rGAD2/9382] (V5589)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V5589-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 559 | |
|
|
V5589-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 259 | |
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V5589SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Rabbit |
| Clonality | Recombinant Mouse Monoclonal |
| Isotype | Rabbit IgG, kappa |
| Clone Name | rGAD2/9382 |
| Purity | Protein A/G affinity |
| UniProt | Q05329 |
| Localization | Cytoplasm |
| Applications | Immunohistochemistry (FFPE) : 1-2ug/ml |
| Limitations | This GAD2 antibody is available for research use only. |
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Glutamate decarboxylase 2 is a pyridoxal phosphate-dependent enzyme encoded by the GAD2 gene and commonly referred to as GAD65. The GAD2 Antibody Recombinant Mouse MAb clone rGAD2/9382 is developed to detect this key GABA-synthesizing enzyme in central nervous system and neuroendocrine research applications. GAD2 is located on chromosome 10p11.23 and encodes the 65 kDa isoform of glutamate decarboxylase responsible for catalyzing the conversion of glutamate to gamma-aminobutyric acid, the principal inhibitory neurotransmitter in the brain.
GAD65 is predominantly expressed in GABAergic neurons, where it localizes to the cytoplasm and associates with synaptic vesicle membranes. Unlike the related isoform GAD1, which produces GAD67 and contributes to basal GABA synthesis, GAD2 is more closely linked to activity-dependent neurotransmitter production and synaptic release. In tissue-based studies, immunohistochemical detection typically demonstrates cytoplasmic staining within neuronal cell bodies and proximal processes, particularly in cortex, hippocampus, cerebellum, and other regions enriched for inhibitory interneurons. Non-neuronal tissues generally show minimal staining, reflecting the restricted neuronal distribution of GAD65.
In neuropathology research, altered GAD2 expression has been examined in epilepsy, neurodevelopmental disorders, and neurodegenerative disease, where disruptions in excitatory-inhibitory balance are central to disease mechanisms. GAD65 is also a well-characterized autoantigen in type 1 diabetes and certain neurologic autoimmune syndromes, further supporting its importance in both neurologic and immunologic research contexts. Detection of GAD2 supports studies mapping inhibitory circuitry and analyzing changes in GABAergic neuron populations.
As a central enzyme in GABA biosynthesis, GAD2 plays a critical role in maintaining synaptic inhibition and neural network stability. Clone rGAD2/9382 is a recombinant mouse monoclonal antibody developed for specific detection of GAD65 in tissue sections and other protein expression studies, supporting research focused on inhibitory neuron identification and characterization of GABAergic pathways.
Optimal dilution of the GAD2 antibody should be determined by the researcher.
A recombinant human GAD2 /GAD65 protein fragment (within amino acids 1-200) was used as the immunogen for the GAD2 antibody recombinant mouse mAb rGAD2/9382.
Aliquot the GAD2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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