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Home >> Antibodies >> Recombinant FTL Antibody / Ferritin Light Chain

Recombinant FTL Antibody / Ferritin Light Chain [clone rFTL/1386] (V8510)

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  Catalog No Formulation Size Price (USD)  
Image V8510-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V8510-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V8510SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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Western blot testing of human kidney lysate with recombinant FTL antibody. Predicted molecular weight: ~20 kDa.
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Western blot testing of human kidney lysate with recombinant FTL antibody. Predicted molecular weight: ~20 kDa.
IHC staining of FFPE human kidney with recombinant FTL antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant FTL antibody as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rFTL/1386
Purity Protein G affinity chromatography
UniProt P02792
Localization Cytoplasmic
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This recombinant FTL antibody is available for research use only.
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Description

Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts.

Application Notes

Optimal dilution of the recombinant FTL antibody should be determined by the researcher.

Immunogen

Recombinant full-length human FTL protein was used as the immunogen for the recombinant FTL antibody.

Storage

Store the recombinant FTL antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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