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Home >> Antibodies >> Recombinant FSP1 Antibody / S100A4

Recombinant FSP1 Antibody / S100A4 [clone rS100A4/1481] (V8068)

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  Catalog No Formulation Size Price (USD)  
Image V8068-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V8068-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V8068SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC testing of FFPE human placenta with FSP1 antibody (clone rS100A4/1481). Required HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling.
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IHC testing of FFPE human placenta with FSP1 antibody (clone rS100A4/1481). Required HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling.
IHC testing of FFPE human placenta with FSP1 antibody (clone rS100A4/1481). Required HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling.
IF/ICC testing of fixed and permeabilized human A549 cells with FSP1 antibody (clone rS100A4/1481, green) and Reddot nuclear stain (red).
Flow cytometry testing of permeabilized human A549 cells with FSP1 antibody (clone rS100A4/1481); Red=isotype control, Blue= FSP1 antibody.
Western blot testing of human PANC-1 cell lysate with FSP1 antibody. Predicted molecular weight ~12 kDa.
SDS-PAGE analysis of purified, BSA-free recombinant FSP1 antibody (clone rS100A4/1481) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rS100A4/1481
Purity Protein G affinity chromatography
UniProt P26447
Localization Cytoplasmic, nuclear
Applications Flow cytometry : 1-2ug/10^6 cells in 0.1ml
Western blot : 1-2ug/ml
Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant FSP1 antibody is available for research use only.
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Description

S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, 64 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.

Application Notes

Optimal dilution of the recombinant FSP1 antibody should be determined by the researcher.

Immunogen

A recombinant human partial protein (amino acids 1-200) was used as the immunogen for the recombinant FSP1 antibody.

Storage

Store the recombinant FSP1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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