FOXA1 Antibody / Developmental Transcription Factor Antibody [clone FOXA1/2230R] (V3620)

FOXA1 Antibody MCF7 Cell IF. Immunofluorescent staining of PFA-fixed human MCF7 cells using FOXA1 Antibody / Developmental Transcription Factor Antibody (clone FOXA1/2230R) demonstrates strong green nuclear staining consistent with localization of FOXA1, a DNA-binding transcription factor that regulates developmental gene expression, epithelial differentiation, and tissue-specific transcriptional programs. The predominantly nuclear staining pattern is characteristic of a transcriptional regulator and supports the role of FOXA1 in controlling gene networks that govern cellular identity and differentiation. Phalloidin (red) labels the actin cytoskeleton and outlines cellular morphology, providing structural context for the nuclear localization of FOXA1.

FOXA1 Antibody Human Tonsil IHC. Immunohistochemical staining of FFPE human tonsil tissue using FOXA1 Antibody / Developmental Transcription Factor Antibody (clone FOXA1/2230R) demonstrates strong nuclear HRP-DAB brown staining within epithelial cell populations lining glandular and crypt-associated structures. The staining pattern is consistent with expression of FOXA1, a forkhead family transcription factor that regulates developmental gene expression, epithelial differentiation, and tissue-specific transcriptional programs. The predominantly nuclear localization is characteristic of a DNA-binding transcriptional regulator and supports the role of FOXA1 in maintaining epithelial cellular identity and differentiated tissue phenotypes. HIER: boil sections in 10 mM Tris with 1 mM EDTA, pH 9 for 10-20 minutes followed by cooling at room temperature for 20 minutes.

FOXA1 Antibody HeLa Cell Western Blot. Western blot analysis of human HeLa cell lysate using FOXA1 Antibody / Developmental Transcription Factor Antibody (clone FOXA1/2230R) demonstrates a distinct band at approximately 49 kDa, consistent with the predicted molecular weight of FOXA1. FOXA1 is a forkhead family transcription factor and pioneer regulator that facilitates chromatin accessibility and controls gene expression programs involved in embryonic development, organogenesis, epithelial differentiation, and tissue-specific cellular identity. Detection of the expected molecular weight band supports expression of FOXA1 and its role as a developmental transcription factor that coordinates lineage-specific transcriptional networks and differentiated cellular phenotypes.

FOXA1 Antibody MCF-7 Cell FACS. Flow cytometry analysis of PFA-fixed human MCF-7 cells using FOXA1 Antibody / Developmental Transcription Factor Antibody (clone FOXA1/2230R) demonstrates a pronounced rightward shift of the FOXA1-positive population (blue) relative to the isotype control (red), consistent with expression of FOXA1. FOXA1 is a forkhead family transcription factor and pioneer regulator that controls developmental gene expression, epithelial differentiation, chromatin accessibility, and tissue-specific transcriptional programs. As a developmental transcription factor, FOXA1 plays a central role in establishing cellular identity and regulating lineage-specific gene expression networks. Red=isotype control; Blue=FOXA1 antibody.

SDS-PAGE analysis of purified, BSA-free recombinant FOXA1 antibody (clone FOXA1/2230R) as confirmation of integrity and purity.