• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Home >> Antibodies >> FLI1 Antibody

FLI1 Antibody [clone FLI1/8318R] (V4210)

  Catalog No Formulation Size Price (USD)  
Image V4210-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 559
Image
V4210-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 259
Image
V4210SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
Microvalidated Recrabbitmono
Bulk quote request
FLI1 Antibody Ramos WB. Western blot analysis of Ramos cell lysate using FLI1 Antibody clone FLI1/8318R. A prominent band is detected at approximately 50-55 kDa, consistent with the expected molecular weight range of Friend leukemia integration 1 transcription factor / FLI1, a nuclear ETS family regulatory protein involved in hematopoietic differentiation and transcriptional control pathways.
SDS-PAGE analysis of purified, BSA-free recombinant FLI1 antibody (clone FLI1/8318R) as confirmation of integrity and purity.
FLI1 Antibody Lymph Node IHC. Immunohistochemistry analysis of FFPE human lymph node tissue stained with recombinant FLI1 antibody (clone FLI1/8318R). Strong nuclear HRP-DAB brown staining is observed throughout lymphoid cell populations, consistent with the expected nuclear localization of Friend leukemia integration 1 transcription factor / FLI1, an ETS family transcription-associated regulatory protein involved in hematopoietic differentiation and immune cell gene expression pathways. Sections underwent HIER using 10mM Tris with 1mM EDTA buffer, pH 9, prior to immunostaining.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name FLI1/8318R
Purity Protein A affinity
UniProt Q01543
Localization Nucleus
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Western Blot : 2-4ug/ml
Limitations This FLI1 antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

Description

FLI1 Antibody recognizes a protein of 51kDa, which is identified as Friend leukemia integration 1 transcription factor. This protein, a member of the ETS family of DNA binding transcription factors, is involved in cellular proliferation and tumorigenesis. Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. Members of the Ets gene family share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. Approximately 90% of Ewing s Sarcoma (EWS) / Primitive Neuroectodermal Tumors (PNET) have a specific translocation, t(11;22)(q24;q12), which results in fusion of EWS to Fli-1, and production of an EWS-Fli-1 fusion protein. Among normal tissues only endothelial cells and small lymphocytes express Fli-1. This protein is expressed in majority of vascular tumors including angiosarcomas, hemangioendotheliomas, hemangiomas, and Kaposi s Sarcomas. High sensitivity and specificity of Fli-1 equals to or exceeds that of the established vascular markers like CD31, CD34, and Factor VIII.

Explore our FLI1 Antibody / ETS Transcription Factor Marker page for additional validation data and research applications involving Ewing sarcoma biology, hematopoietic differentiation, endothelial signaling, and ETS family transcriptional regulation pathways.

Application Notes

Optimal dilution of the FLI1 antibody should be determined by the researcher.

Immunogen

A recombinant partial protein (within amino acids 200-400) from the human protein was used as the immunogen for the recombinant FLI1 antibody.

Storage

Aliquot the FLI1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.