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Home >> Antibodies >> Recombinant Filaggrin Antibody

Recombinant Filaggrin Antibody [clone FLG/3909R] (V9401)

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  Catalog No Formulation Size Price (USD)  
Image V9401-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9401-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9401SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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Western blot analysis of human A431 cell lysate using recombinant Filaggrin antibody (clone FLG/3909R). Expected molecular weight: ~434 kDa (precursor); 37-45 kDa (processed).
Western blot analysis of human A431 cell lysate using recombinant Filaggrin antibody (clone FLG/3909R). Expected molecular weight: ~434 kDa (precursor); 37-45 kDa (processed).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name FLG/3909R
Purity Protein A/G affinity
UniProt P20930
Localization Cytoplasm
Applications Western blot : 2-4ug/ml
Limitations This recombinant Filaggrin antibody is available for research use only.
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Description

Filaggrin is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules.Active filaggrin is present at a level of the epidermis where keratinocytes are in transition between the live nucleated granular layer and the anucleate cornified layer, suggesting that filaggrin aids in the terminal differentiation process by facilitating apoptotic machinery.

Application Notes

Optimal dilution of the recombinant Filaggrin antibody should be determined by the researcher.

Immunogen

A portion of amino acids 198-288 was used as the immunogen for the recombinant Filaggrin antibody.

Storage

Aliquot the recombinant Filaggrin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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