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Home >> Antibodies >> Recombinant Ferritin Light Chain Antibody / Rabbit Monoclonal

Recombinant Ferritin Light Chain Antibody / Rabbit Monoclonal [clone FTL/2338R] (V3569)

  Catalog No Formulation Size Price (USD)  
Image V3569-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3569-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3569SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V3569IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Microvalidated Recrabbitmono
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IHC testing of FFPE human breast carcinoma with recombinant Ferritin Light Chain antibody (clone FTL/2338R). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free recombinant Ferritin Light Chain antibody (clone FTL/2338R) as confirmation of integrity and purity.
Western blot testing of human HeLa lysate with Ferritin Light Chain antibody. Predicted molecular weight: ~20 kDa.
Western blot testing of human kidney lysate with Ferritin Light Chain antibody. Predicted molecular weight: ~20 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name FTL/2338R
Purity Protein A affinity chromatography
UniProt P02792
Localization Cytoplasmic
Applications Western blot : 0.5-2ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Limitations This recombinant Ferritin Light Chain antibody is available for research use only.
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Description

The FTL gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. This gene has multiple pseudogenes.

Although ferritin light chain has no ferroxidase activity, the light chain may be responsible for the electron transfer across the ferritin protein cage. [Wiki]

Application Notes

Optimal dilution of the recombinant Ferritin Light Chain antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Amino acids 38-165 of human FTL1 were used as the immunogen for this recombinant Ferritin Light Chain antibody.

Storage

Store the recombinant Ferritin Light Chain antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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