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Home >> Antibodies >> Recombinant EpCAM Antibody

Recombinant EpCAM Antibody [clone rEGP40/4547] (V8650)

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  Catalog No Formulation Size Price (USD)  
Image V8650-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V8650-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V8650SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human colon carinoma with recombinant EpCAM antibody (clone rEGP40/4547). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human colon carinoma with recombinant EpCAM antibody (clone rEGP40/4547). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon carinoma with recombinant EpCAM antibody (clone rEGP40/4547). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant EpCAM antibody (clone rEGP40/4547) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG2a, kappa
Clone Name rEGP40/4547
Purity Protein G affinity chromatography
UniProt P16422
Localization Cell surface, cytoplasmic
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This recombinant EpCAM antibody is available for research use only.
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Description

EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Application Notes

Optimal dilution of the recombinant EpCAM antibody should be determined by the researcher.

Immunogen

Recombinant full-length human EpCAM protein was used as the immunogen for the recombinant EpCAM antibody. The binding epitope of this antibody is located in the first EGF-like repeat domain (EGF1) between amino acids 27-59.

Storage

Store the recombinant EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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