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Home >> Antibodies >> Recombinant EpCAM Antibody / Cytoplasmic domain

Recombinant EpCAM Antibody / Cytoplasmic domain [clone EGP40/7035R] (V8850)

  Catalog No Formulation Size Price (USD)  
Image V8850-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V8850-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V8850SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human colon tissue with recombinant EpCAM antibody (clone EGP40/7035R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human brain tissue with recombinant EpCAM antibody (clone EGP40/7035R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant EpCAM antibody (EGP40/7035R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name EGP40/7035R
Purity Protein A affinity
UniProt P16422
Localization Cell surface, cytoplasmic
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant EpCAM antibody is available for research use only.
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Description

Epithelial cellular adhesion molecule (Ep-CAM), also called epithelial specific antigen (ESA), is a 40-43kDa transmembrane epithelial glycoprotein. It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Application Notes

Optimal dilution of the recombinant EpCAM antibody should be determined by the researcher.

Immunogen

A portion of amino acids 289-314 of human EpCAM was used as the immunogen for the recombinant EpCAM antibody.

Storage

Aliquot the recombinant EpCAM antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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