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Home >> Antibodies >> Recombinant EpCAM Antibody

Recombinant EpCAM Antibody [clone EGP40/2571R] (V8217)

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  Catalog No Formulation Size Price (USD)  
Image V8217-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V8217-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V8217SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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Flow cytometry testing of human MCF7 cells with recombinant EpCAM antibody (clone EGP40/2571R); Red=isotype control, Blue= recombinant EpCAM antibody.
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Flow cytometry testing of human MCF7 cells with recombinant EpCAM antibody (clone EGP40/2571R); Red=isotype control, Blue= recombinant EpCAM antibody.
IHC staining of FFPE human prostate carcinoma with recombinant EpCAM antibody (clone EGP40/2571R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC staining of FFPE human colon carcinoma with recombinant EpCAM antibody (clone EGP40/2571R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant EpCAM antibody (clone EGP40/2571R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name EGP40/2571R
Purity Protein A affinity chromatography
UniProt P16422
Localization Cell surface, cytoplasmic
Applications Flow cytometry : 1-2ug/10^6 cells in 0.1ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant EpCAM antibody is available for research use only.
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Description

EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Application Notes

Optimal dilution of the recombinant EpCAM antibody should be determined by the researcher.

Immunogen

A recombinant human partial protein (amino acids 100-224) was used as the immunogen for the EpCAM antibody. The epitope of this mAb has been mapped to between amino acids 202-212.

Storage

Store the recombinant EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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