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Home >> Antibodies >> Recombinant EpCAM Antibody / Cytoplasmic domain

Recombinant EpCAM Antibody / Cytoplasmic domain [clone rEGP40/1110] (V3619)

  Catalog No Formulation Size Price (USD)  
Image V3619-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3619-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3619SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V3619IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Microvalidated Recmousemono
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IHC testing of FFPE human ovarian carcinoma with recombinant EpCAM antibody (clone rEGP40/1110). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free recombinant EpCAM antibody (clone rEGP40/1110) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using recombinant EpCAM antibody (clone rEGP40/1110). These results demonstrate the foremost specificity of the rEGP40/1110 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1
Clone Name rEGP40/1110
Purity Protein G affinity chromatography
UniProt P16422
Localization Cell surface, cytoplasmic
Applications Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Limitations This recombinant EpCAM antibody is available for research use only.
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Description

EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Application Notes

Optimal dilution of the recombinant EpCAM antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A portion of amino acids 289-314 (cytoplasmic domain) was used as the immunogen for the recombinant EpCAM antibody.

Storage

Store the recombinant EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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