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Home >> Antibodies >> Recombinant Ep-CAM Antibody / Rabbit Monoclonal

Recombinant Ep-CAM Antibody / Rabbit Monoclonal [clone EGP40/1556R] (V7265)

  Catalog No Formulation Size Price (USD)  
Image V7265-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V7265-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V7265SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
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V7265IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 429
IHC testing of FFPE human colon carcinoma and recombinant Ep-CAM antibody (clone EGP40/1556R). Required HIER: steam sections in pH6 citrate buffer for 10-20 min.
SDS-PAGE analysis of purified, BSA-free recombinant EpCAM antibody (clone EGP40/1556R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human, Rat
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name EGP40/1556R
Purity Protein A affinity chromatography
UniProt P16422
Localization Cell surface, cytoplasmic
Applications Flow cytometry : 1-2ug/10^6 cells
Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Limitations This recombinant Ep-CAM antibody is available for research use only.
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Description

Ep-CAM may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. [UniProt]

Application Notes

Titering of the recombinant Ep-CAM antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Human protein was used as the immunogen for the recombinant Ep-CAM antibody.

Storage

Store the recombinant Ep-CAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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